Goldenberg H, Hüttinger M, Kampfer P, Kramar R
Histochem J. 1978 Jan;10(1):103-13. doi: 10.1007/BF01003417.
Peroxisomes from carp liver can be separated by isopycnic density gradient centrifugation in sucrose. Without reaching complete sedimentation equilibrium, the purification by this method is quite successful. There is a 40-fold enrichment of catalase, the peroxisomal marker, with a total yield of 27%. No pretreatment of animals is necessary for separation from lysosomes, which, besides high fragility, show lower buoyant densities than peroxisomes. The enzyme content of carp liver peroxisomes is similar to that of rat liver, with the exception of alpha-glycerophosphate dehydrogenase, which in this tissue is a completely soluble cytoplasmic enzyme. Total activities are much lower than in the rat, for the characteristic peroxisomal oxidases the difference being in the range of one order of magnitude.
鲤鱼肝脏中的过氧化物酶体可通过在蔗糖中进行等密度梯度离心来分离。在未达到完全沉降平衡的情况下，用这种方法进行纯化相当成功。过氧化物酶体标记物过氧化氢酶的富集倍数为40倍，总产率为27%。从溶酶体中分离不需要对动物进行预处理，溶酶体除了高度易碎外，其浮力密度比过氧化物酶体低。鲤鱼肝脏过氧化物酶体的酶含量与大鼠肝脏相似，但α-甘油磷酸脱氢酶除外，该酶在这种组织中是一种完全可溶的细胞质酶。总活性远低于大鼠，特征性过氧化物酶体氧化酶的差异在一个数量级范围内。
