Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
J Altern Complement Med. 2013 Jul;19(7):671-6. doi: 10.1089/acm.2012.0625. Epub 2013 Jan 30.
This present work was aimed to investigate wound healing-related biologic activities of traditional herbal formulas used for wound treatment in southern Thailand.
Water and ethanol extracts of the formulas (THR-SK004, THR-SK010, and THR-SK011) were tested for their antibacterial potency against methicillin-resistant Staphylococcus aureus (MRSA) and -susceptible S. aureus. Anti-inflammatory activities of the extracts were assessed by detection of the inhibition of lipopolysaccharide-induced nitric oxide production. Anti-oxidant activities and cytotoxicity of the extracts were also measured.
Among the tested formulas, ethanol extract of THR-SK010 consisting of four herbs: Curcuma longa L., Areca catechu L., Oryza sativa L., and Garcinia mangostana L., was found to possess promising antibacterial activities with MIC90 of 4 μg mL(-1) against MRSA isolates. This ethanol extract offered the highest anti-inflammatory activity as well as DPPH and hydroxyl radical scavenging activities.
Remarkable antibacterial, anti-inflammatory, and antioxidant activities as well as low toxicity on Vero cells of THR-SK010 ethanol extract provide scientific information to support the topical use of the formula for wound treatment. This information proposes the potential to develop a new generation of phytopharmaceuticals based on traditional knowledge.
本研究旨在探索泰国南部用于治疗创伤的传统草药配方的伤口愈合相关生物学活性。
测试了配方（THR-SK004、THR-SK010 和 THR-SK011）的水和乙醇提取物对耐甲氧西林金黄色葡萄球菌（MRSA）和敏感金黄色葡萄球菌的抗菌效力。通过检测脂多糖诱导的一氧化氮产生的抑制作用来评估提取物的抗炎活性。还测量了提取物的抗氧化活性和细胞毒性。
在所测试的配方中，由四种草药组成的 THR-SK010 的乙醇提取物：姜黄、槟榔、稻和藤黄，对 MRSA 分离株具有有前景的抗菌活性，MIC90 为 4μgmL（-1）。该乙醇提取物具有最高的抗炎活性以及 DPPH 和羟基自由基清除活性。
THR-SK010 乙醇提取物具有显著的抗菌、抗炎和抗氧化活性，对 Vero 细胞的毒性低，为支持该配方用于创伤治疗提供了科学信息。这一信息提出了基于传统知识开发新一代植物药的潜力。
