School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
Centre for Freshwater Management, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.
J Gen Virol. 2013 May;94(Pt 5):1104-1110. doi: 10.1099/vir.0.050088-0. Epub 2013 Jan 30.
Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes micro-organisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.
在过去的几年中，人们发现了高度多样化的新型 ssDNA 病毒。在这里，我们介绍了第一个 ssDNA 病毒，即从腹足类动物 Amphibola crenata Martyn 1784 中回收的 Gastropod-associated circular ssDNA virus (GaCSV)。该腹足类动物是一种沉积食者，以在潮汐泥滩表面放牧微生物和有机碎屑为生。GaCSV（2351nt）基因组包含两个双向转录的 ORF。较小的 ORF（874nt）与某些细菌和圆环病毒的病毒复制相关蛋白（Rep）序列具有相似性，而较大的 ORF（955nt）与公共数据库中的任何序列均不相关，我们推测它可能编码衣壳蛋白。系统发育分析表明，GaCSV 的 Rep 与细菌来源的 Rep 样序列聚类，突出了 ssDNA 病毒在水平基因转移中的作用。在与人类污染有关的生物体中出现以前未知的病毒是一个相对未被探索的领域。
