State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
J Med Virol. 2013 Apr;85(4):646-54. doi: 10.1002/jmv.23490. Epub 2013 Jan 30.
The effectiveness of the E2/E6 ratio, the state of viral genome integration and the viral load of human papillomavirus 16 (HPV 16) in predicting the risk of cervical cancer among Chinese women was investigated. Quantitative PCRs for the E2/E6 ratio and the viral load were performed on 85 cervical cancer samples and 55 HPV 16 positive healthy controls. The integrated form of the viral genome was found in 10.9% control samples and in 26.4% cervical cancer samples (P = 0.02). The majority of the cervical cancer (63.2%) and control samples (60%) were mixed forms. The E2/E6 ratio was associated with a high risk of cervical cancer (OR = 7.29, P = 9.55E-6). The integrated form (OR = 6.54, P = 0.005) and mixed form (OR = 2.93, P = 0.042) increased the risk of cervical cancer. The mean viral load in cervical cancer samples (37,371 ± 227,135) was higher than that in the controls (4,619 ± 27,079; P = 0.011). Additionally, the viral load increased along with the cervical cancer progression from the International Federation of Gynecology and Obstetrics (FIGO) stage I (12,337 ± 25,604) to stage II (67,453 ± 319,821). Compared with the state of viral genome integration (area under the receiver operating characteristic curve (AUC) = 0.743) or the viral load (AUC = 0.694), the E2/E6 ratio improved the effectiveness of the risk prediction of cervical cancer (AUC = 0.777), with the sensitivity (specificity) 81.2% (71.7%). The state of viral genome integration and the viral load of HPV 16 were important factors for the risk prediction of cervical cancer among Chinese women, and the E2/E6 ratio had a better cervical cancer risk prediction with age adjustment.
研究了 E2/E6 比值、病毒基因组整合状态和人乳头瘤病毒 16（HPV16）病毒载量在预测中国女性宫颈癌风险中的作用。对 85 例宫颈癌样本和 55 例 HPV16 阳性健康对照进行了 E2/E6 比值和病毒载量定量 PCR。在 10.9%的对照样本和 26.4%的宫颈癌样本中发现了病毒基因组的整合形式（P=0.02）。大多数宫颈癌（63.2%）和对照样本（60%）为混合形式。E2/E6 比值与宫颈癌高风险相关（OR=7.29，P=9.55E-6）。整合形式（OR=6.54，P=0.005）和混合形式（OR=2.93，P=0.042）增加了宫颈癌的风险。宫颈癌样本的平均病毒载量（37371±227135）高于对照组（4619±27079；P=0.011）。此外，病毒载量随着宫颈癌从国际妇产科联合会（FIGO）I 期（12337±25604）到 II 期（67453±319821）的进展而增加。与病毒基因组整合状态（受试者工作特征曲线下面积（AUC）=0.743）或病毒载量（AUC=0.694）相比，E2/E6 比值提高了宫颈癌风险预测的有效性（AUC=0.777），其灵敏度（特异性）为 81.2%（71.7%）。HPV16 病毒基因组整合状态和病毒载量是中国女性宫颈癌风险预测的重要因素，E2/E6 比值在年龄调整后对宫颈癌风险有更好的预测作用。
