Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Mol Biol Rep. 2024 Mar 11;51(1):411. doi: 10.1007/s11033-024-09397-6.
This study examined the viral load and physical status of the human papillomavirus 16 (HPV-16) genome in non-cancerous, precancerous and cancerous cervical lesions.
Quantitative real-time PCR was performed to determine HPV-16 E2 and E6 viral load in 132 cervical specimens. E2/E6 viral load ratio was used to determine the physical status of HPV-16 genome.
E2 gene viral load was a significant (P < 0.001) predicting biomarker in differentiating non-cancerous from precancerous and cancerous samples. E6 gene viral load was significantly different between the groups (P < 0.001). The specificity and sensitivity of E2 and E6 in distinguishing SCC samples were 100% and 95% respectively.
HPV-16 viral load measured through E2 and E6 genes is a reliable indicator of lesion type.
本研究检测了非癌性、癌前病变和癌性宫颈病变中人乳头瘤病毒 16（HPV-16）基因组的病毒载量和物理状态。
采用实时定量 PCR 法检测 132 例宫颈标本中 HPV-16 E2 和 E6 的病毒载量。E2/E6 病毒载量比用于确定 HPV-16 基因组的物理状态。
E2 基因病毒载量是区分非癌性与癌前病变和癌症样本的显著（P<0.001）预测生物标志物。E6 基因病毒载量在各组之间有显著差异（P<0.001）。E2 和 E6 区分 SCC 样本的特异性和敏感性分别为 100%和 95%。
通过 E2 和 E6 基因测量的 HPV-16 病毒载量是病变类型的可靠指标。
