Laboratory of Plant Proteins, Faculty of Exact Sciences, National University of La Plata, La Plata, Argentina.
Planta Med. 2013 Mar;79(3-4):207-13. doi: 10.1055/s-0032-1328201. Epub 2013 Jan 30.
Some plant proteases (e. g., papain, bromelain, ficin) have been used as anti-inflammatory agents for some years, and especially bromelain is still being used as alternative and/or complementary therapy to glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. Bromelain is an extract rich in cysteine endopeptidases obtained from Ananas comosus. In this study the anti-inflammatory action of a partially purified extract of Bromelia hieronymi fruits, whose main components are cysteine endopeptidases, is presented. Different doses of a partially purified extract of B. hieronymi were assayed on carrageenan-induced and serotonine-induced rat paw edema, as well as in cotton pellet granuloma model. Doses with equal proteolytic activity of the partially purified extract and bromelain showed significantly similar anti-inflammatory responses. Treatment of the partially purified extract and bromelain with E-64 provoked loss of anti-inflammatory activity on carrageenan-induced paw edema, a fact which is consistent with the hypothesis that the proteolytic activity would be responsible for the anti-inflammatory action.
一些植物蛋白酶（例如木瓜蛋白酶、菠萝蛋白酶、无花果蛋白酶）多年来一直被用作抗炎剂，特别是菠萝蛋白酶仍被用作糖皮质激素、非甾体类抗风湿药和免疫调节剂的替代和/或补充疗法。菠萝蛋白酶是一种从菠萝中提取的富含半胱氨酸内肽酶的提取物。在这项研究中，介绍了一种部分纯化的 Bromelia hieronymi 果实提取物的抗炎作用，其主要成分是半胱氨酸内肽酶。不同剂量的部分纯化的 Bromelia hieronymi 提取物在角叉菜胶诱导和 5-羟色胺诱导的大鼠足肿胀以及棉塞肉芽肿模型中进行了检测。部分纯化提取物和菠萝蛋白酶的等蛋白水解活性剂量表现出明显相似的抗炎反应。用 E-64 处理部分纯化提取物和菠萝蛋白酶会导致角叉菜胶诱导的足肿胀的抗炎活性丧失，这一事实与蛋白酶活性负责抗炎作用的假设一致。
