Department of Respiratory Medicine, The Military General Hospital of Beijing, PLA NO.5 Nanmencang, Dongcheng District, Beijing 100700, China.
Yonsei Med J. 2013 Mar 1;54(2):476-9. doi: 10.3349/ymj.2013.54.2.476.
To evaluate the feasibility for gold immunochromatographic assay (GICA) in rapid detection of influenza virus A infection.
Seventy-three patients were enrolled. All patients contributed nasopharyngeal secretions and paired serum samples. Nasopharyngeal secretions was used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen. Paired serum samples were used for the hemagglutination inhibition assay at the Centers for Disease Control and Prevention influenza network laboratory in Beijing.
Compare GICA test to hemagglutination inhibition (HI) assay, the Kappa value was 0.402 and the p value in the paired χ² test was higher than 0.05. Therefore, the difference was not statistically significant. The sensitivity of GICA was 50.0% and the specificity was 90.2%, and the negative predictive value was 90.2%.
The sensitivity for Influenza A antigen detection by using GICA is relatively low, the specificity is relatively satisfactory.
评估金免疫层析法（GICA）快速检测甲型流感病毒感染的可行性。
共纳入 73 例患者。所有患者均采集鼻咽分泌物和配对血清样本。标本采集后立即使用胶体金免疫层析快速检测甲型流感病毒的鼻咽分泌物。配对血清样本用于北京疾病预防控制中心流感网络实验室的血凝抑制检测。
与血凝抑制（HI）检测相比，GICA 试验的 Kappa 值为 0.402，配对 χ²检验的 p 值大于 0.05。因此，差异无统计学意义。GICA 的灵敏度为 50.0%，特异性为 90.2%，阴性预测值为 90.2%。
GICA 检测甲型流感病毒抗原的灵敏度相对较低，特异性较为满意。
