Jung K, Scholz M, Fahlke J
Department of Experimental Organ Transplantation, University Hospital Charité, Humboldt University Berlin, German Democratic Republic.
Transplantation. 1990 May;49(5):890-4. doi: 10.1097/00007890-199005000-00011.
The role of hypothermic storage of rat kidneys on lipid peroxidation compared to its effect on mitochondrial respiratory parameters has been investigated. Rat kidneys were flushed with cold solutions of isotonic sodium chloride; Euro-Collins'; preservation solution containing histidine buffer, tryptophane, and alpha-ketoglutarate (HTK); or hypertonic citrate and then stored for 20 hr at 4 degrees C. After storage, the endogenous contents of malondialdehyde as well as the chemically (by Fe2+/ascorbic acid) and enzymatically (by Fe3+/ADP/NADPH) induced generation of malondialdehyde were measured in cortical homogenates and partly in mitochondria and microsomes by the thiobarbituric-acid reaction. Compared to the values measured in fresh, unstored kidneys, the levels of malondialdehyde were significantly higher in kidneys preserved in solutions of isotonic sodium chloride or HTK. This stimulating effect of the HTK solution on the generation of lipid peroxidation products could also be established when homogenate was incubated with this solution. Euro-Collins' and hypertonic citrate solution did not change the endogenous contents of malondialdehyde in kidneys during hypothermic storage. Both the chemically and enzymatically induced lipid peroxidation increased after hypothermic storage of kidneys in all solutions investigated. No direct relationship between the contents of malondialdehyde and respiratory mitochondrial parameters was detectable. The results demonstrate that the extent of lipid peroxidation does not correlate with preservation effectiveness.
研究了大鼠肾脏低温保存对脂质过氧化的作用，并与它对线粒体呼吸参数的影响进行了比较。用等渗氯化钠冷溶液、含有组氨酸缓冲液、色氨酸和α-酮戊二酸的Euro-Collins保存液（HTK）或高渗柠檬酸盐冲洗大鼠肾脏，然后在4℃下保存20小时。保存后，通过硫代巴比妥酸反应在皮质匀浆中以及部分在线粒体和微粒体中测量丙二醛的内源性含量以及化学诱导（通过Fe2+/抗坏血酸）和酶促诱导（通过Fe3+/ADP/NADPH）产生的丙二醛。与新鲜未保存的肾脏中测得的值相比，等渗氯化钠溶液或HTK保存的肾脏中丙二醛水平显著更高。当匀浆与该溶液一起孵育时，也可确定HTK溶液对脂质过氧化产物生成的这种刺激作用。在低温保存期间，Euro-Collins溶液和高渗柠檬酸盐溶液未改变肾脏中丙二醛的内源性含量。在所有研究的溶液中，肾脏低温保存后化学诱导和酶促诱导的脂质过氧化均增加。未检测到丙二醛含量与线粒体呼吸参数之间的直接关系。结果表明，脂质过氧化程度与保存效果无关。
