Illsley N P, Aarnoudse J G, Penfold P, Bardsley S E, Coade S B, Stacey T E, Hytten F E
Placenta. 1984 May-Jun;5(3):213-25. doi: 10.1016/s0143-4004(84)80031-4.
In vitro dual circuit perfusion of the placenta with well-oxygenated medium results in the continuous and stable consumption of oxygen and glucose over a 2-h perfusion period. This is reflected in a stable production of lactate and an energy charge which is higher at the end of the perfusion period than that seen in fresh placental tissue immediately after vaginal delivery. Anoxic perfusion causes an increase in glucose consumption which is more than twofold higher than that seen in the oxygenated perfusion, resulting finally in placental uptake of glucose not only from the maternal but also from the fetal circulation. Lactate production is increased during the anoxic perfusion, while the final tissue energy charge value lies between the values observed for fresh tissue and for the oxygenated perfusion. The shift to anaerobic metabolism shown by placental tissue in anoxic conditions enables continued functioning of the tissue over the 2-h perfusion period but it appears that under anoxic conditions the tissue may incur an energy debt not observed in oxygenated perfusions.
用充分氧合的培养基对胎盘进行体外双循环灌注，在2小时的灌注期内会导致氧气和葡萄糖的持续稳定消耗。这体现在乳酸的稳定产生以及能量电荷上，灌注期结束时的能量电荷高于阴道分娩后新鲜胎盘组织中的能量电荷。缺氧灌注会导致葡萄糖消耗增加，比氧合灌注时高出两倍多，最终导致胎盘不仅从母体循环而且从胎儿循环中摄取葡萄糖。缺氧灌注期间乳酸产生增加，而最终组织能量电荷值介于新鲜组织和氧合灌注所观察到的值之间。胎盘组织在缺氧条件下向无氧代谢的转变使得组织在2小时的灌注期内能够持续发挥功能，但似乎在缺氧条件下组织可能会产生氧合灌注中未观察到的能量亏空。
