Yusheng Yao, Chengyue Wang, Zhiying Wang, Guijun Wang
Cleft Palate Craniofac J. 2014 May;51(3):326-33. doi: 10.1597/12-056. Epub 2013 Jan 31.
Objective : The purpose of this study was to evaluate the effect of different mechanical forces on the expansion of the palatine suture using transsutural distraction osteogenesis. Methods : A total of 48 dogs were used in this study. The experimental groups were treated with a custom-designed internal distractor. Bone regeneration was determined with x-rays and histology. The computed values underwent statistical analyses using analysis of variance. Results : The maxillary complex was most noticeably advanced with an applied mechanical force of 600 g (20.15 ± 1.36 mm), compared with forces of 400 g (19.88 ± 1.41 mm) and 800 g (2.24 ± 0.93 mm). Immunohistochemical staining showed that the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 fluctuated with different mechanical forces. These changes were statistically significant when 600 g of force was applied within 30 days of distraction (P < .05). Conclusions : Transsutural distraction osteogenesis in the growing dog should be safe and well tolerated in inducing bony lengthening of the maxilla, and the optimal force is 600 × g. Bone morphogenetic protein-2 and bone morphogenetic protein-4 may play an important roles in the signaling pathways that link mechanical forces and biological responses.
本研究旨在评估使用经缝牵引成骨术时不同机械力对腭中缝扩展的影响。方法：本研究共使用48只犬。实验组采用定制的内部牵张器进行治疗。通过X射线和组织学确定骨再生情况。对计算值进行方差分析的统计分析。结果：与400 g（19.88±1.41 mm）和800 g（2.24±0.93 mm）的力相比，施加600 g的机械力时上颌复合体前移最为明显（20.15±1.36 mm）。免疫组织化学染色显示，骨形态发生蛋白-2和骨形态发生蛋白-4的表达随不同机械力而波动。在牵引30天内施加600 g力时，这些变化具有统计学意义（P <.05）。结论：在生长中的犬中，经缝牵引成骨术在诱导上颌骨延长方面应是安全且耐受性良好的，最佳力为600×g。骨形态发生蛋白-2和骨形态发生蛋白-4可能在连接机械力和生物学反应的信号通路中起重要作用。
