Rathlavath Srinu, Kohli Vandita, Singh Asem Sanjit, Lekshmi Manjusha, Tripathi Gayatri, Kumar Sanath, Nayak Binaya Bhusan
Post-Harvest Technology, ICAR - Central Institute of Fisheries Education (CIFE), Mumbai 400061, India.
Aquatic Environment and Health Management, ICAR- Central Institute of Fisheries Education (CIFE), Mumbai 400061, India.
Int J Food Microbiol. 2017 Dec 18;263:32-37. doi: 10.1016/j.ijfoodmicro.2017.10.005. Epub 2017 Oct 3.
Arcobacter butzleri is an emerging pathogen isolated from animals, food and the environment. In this study, 147 A. butzleri isolated from seafood and the coastal environment were tested for the presence of ten putative virulence genes (cadF, cj1349, ciaB, mviN, pldA, tlyA, hecA, hecB, irgA, iroE) and antimicrobial susceptibilities. Majority of the isolates harbored mviN (100%), cj1349 (97.2%), ciaB (95.9%), tlyA (91.8%), pldA (91.1%) and cadF (89.7%). Lower detection rates were observed for hecA (10.8%), hecB (19%), iroE (12.9%) and irgA (17.6%). Three A. butzleri isolates harbored all ten virulence genes. The occurrence of cj1349, ciaB, pldA, tlyA and hecA genes was significantly different (P≤0.05) among the isolates from different sources. All (100%) A. butzleri isolates were resistant to vancomycin, cephalothin, cefoxitin and sulphamethizole and susceptible to polymyxin-B, kanamycin, streptomycin, gentamicin, tetracycline and imipenem. Resistance to clinically important antibiotics such as cefotaxime (99.3%), ceftazidime (87.7%), nalidixic acid (70.7%), ampicillin (72.1%), ertapenem and amoxicillin-clavulanic acid (41.9%) was observed in A. butzleri from the environment. The isolates were highly susceptible to norfloxacin (97.9%) and colistin (97.2%), followed by ciprofloxacin (88.4%), meropenem (74.8%), chloramphenicol (72.7%) and erythromycin (69.3%). A. butzleri from different sources were not significantly different with respect to their antimicrobial susceptibility patterns. Multidrug resistance was observed in 66 (81.4%) isolates from fish, 29 (72.5%) isolates from shellfish and 17 (65.3%) isolates from coastal water. A. butzleri harboring virulence genes and resistance to multiple antibiotics found in seafood could be a potential health risk to seafood handlers and consumers. Continuous monitoring of seafood for potentially pathogenic A. butzleri is important to understand the evolution of antibiotic resistance in this emerging food pathogen and to determine the antimicrobial therapy regimen in the event of food-borne A. butzleri infections.
布氏弓形杆菌是一种新出现的病原体，可从动物、食物和环境中分离得到。在本研究中，对从海鲜和沿海环境中分离出的147株布氏弓形杆菌进行了检测，以确定10个假定毒力基因（cadF、cj1349、ciaB、mviN、pldA、tlyA、hecA、hecB、irgA、iroE）的存在情况以及抗菌药敏性。大多数分离株携带mviN（100%）、cj1349（97.2%）、ciaB（95.9%）、tlyA（91.8%）、pldA（91.1%）和cadF（89.7%）。hecA（10.8%）、hecB（19%）、iroE（12.9%）和irgA（17.6%）的检出率较低。有3株布氏弓形杆菌分离株携带所有10个毒力基因。不同来源的分离株中，cj1349、ciaB、pldA、tlyA和hecA基因的出现情况存在显著差异（P≤0.05）。所有（100%）布氏弓形杆菌分离株对万古霉素、头孢噻吩、头孢西丁和磺胺甲噻二唑耐药，对多粘菌素B、卡那霉素、链霉素、庆大霉素、四环素和亚胺培南敏感。从环境中分离出的布氏弓形杆菌对临床重要抗生素如头孢噻肟（99.3%）、头孢他啶（87.7%）、萘啶酸（70.7%）、氨苄西林（72.1%）、厄他培南和阿莫西林-克拉维酸（41.9%）耐药。这些分离株对诺氟沙星（97.9%）和黏菌素（97.2%）高度敏感，其次是环丙沙星（88.4%）、美罗培南（74.8%）、氯霉素（72.7%）和红霉素（69.3%）。不同来源的布氏弓形杆菌在抗菌药敏模式方面无显著差异。在66株（81.4%）来自鱼类的分离株、29株（72.5%）来自贝类的分离株和17株（65.3%）来自沿海水体的分离株中观察到多重耐药。在海鲜中发现携带毒力基因且对多种抗生素耐药的布氏弓形杆菌可能对海鲜加工者和消费者构成潜在健康风险。持续监测海鲜中潜在致病性布氏弓形杆菌对于了解这种新出现的食源性病原体的抗生素耐药性演变以及确定食源性布氏弓形杆菌感染时的抗菌治疗方案很重要。
