Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Universitätsklinikum Magdeburg, Magdeburg, Germany.
Abteilung Mikrobielle Ökologie und Diversitätsforschung, Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
Front Cell Infect Microbiol. 2023 Jan 25;13:1094067. doi: 10.3389/fcimb.2023.1094067. eCollection 2023.
In recent years, has gained clinical significance as an emerging diarrheagenic pathogen associated with poultry and water reservoirs. The full clinical significance of remains rather speculative due to variable virulence and antibiotic susceptibility of individual strains. The aims of the present study were (i) to identify antibiotic resistance genes (ARGs) in the genome sequences of two multidrug-resistant isolates, (ii) to use multilocus-sequence typing (MLST) to generate a guiding phylogeny of isolates collected in Kumasi, Ghana, (iii) to examine the distribution of ARGs in the test cohort, and (iv) to assess the strain's virulence and possible antibiotic treatment options for arcobacteriosis based on the genome sequences and the ARG distribution. A total of 48 A isolates obtained from poultry were included in the analysis. These isolates were genotyped by MLST and the antibiotic susceptibilities of isolates to ampicillin, ciprofloxacin, tetracycline, gentamicin, and erythromycin were tested by disk diffusion. Whole genome sequence data of two multidrug-resistant (MDR) isolates were obtained by a combination of single-molecule real-time (SMRT) and Illumina sequencing technology. A total of 14 ARGs were identified in the two generated genome sequences. For all 48 isolates, the frequency of these 14 ARGs was investigated by PCR or amplicon sequencing. With 44 different sequence types found among 48 isolates, strains were phylogenetically heterogeneous. Four of 48 isolates showed an ARG constellation indicating a multidrug-resistant phenotype. The virulence genes in the two genomes showed that the species might be characterized by a somewhat lower virulence as species. The phenotypic susceptibility data combined with the distribution of the particular ARGs especially and the T81I point mutation of the quinolone resistance determining region (QRDR) in a significant percentage of isolates indicated that macrolides and tetracycline can be recommended for calculated antibiotic treatment of arcobacteriosis in Ghana, but not ampicillin and quinolones.
近年来，作为一种与家禽和水储层有关的新兴致腹泻病原体，在临床上引起了关注。由于个别菌株的毒力和抗生素敏感性不同， 的确切临床意义仍存在很大的推测性。本研究的目的是：(i)鉴定两株多药耐药 分离株基因组序列中的抗生素耐药基因 (ARGs)；(ii) 利用多位点序列分型 (MLST) 构建加纳库马西采集的 分离株的指导进化树；(iii) 研究 ARGs 在测试队列中的分布；(iv) 根据基因组序列和 ARG 分布评估该菌株的毒力和可能的弯曲杆菌病抗生素治疗方案。共纳入 48 株来自家禽的 A 型分离株进行分析。这些分离株通过 MLST 进行基因分型，并通过纸片扩散法检测分离株对氨苄西林、环丙沙星、四环素、庆大霉素和红霉素的药敏性。通过单分子实时 (SMRT) 和 Illumina 测序技术相结合，获得了两株多药耐药 (MDR) 分离株的全基因组序列数据。在两个生成的基因组序列中，共鉴定出 14 个 ARGs。通过 PCR 或扩增子测序，对 48 株分离株的 14 个 ARG 进行了检测。在 48 株分离株中，共发现 44 种不同的序列类型，菌株具有遗传异质性。在 48 株分离株中，有 4 株显示出一种 ARG 组合，表明其表现出多药耐药表型。在两个 基因组中的毒力基因表明，该物种的毒力可能较低。表型药敏数据结合特定 ARGs 的分布，特别是在很大比例的分离株中发现的 81I 点突变的喹诺酮类药物耐药决定区 (QRDR)，表明在加纳，大环内酯类和四环素类可用于计算弯曲杆菌病的抗生素治疗，但不能使用氨苄西林和喹诺酮类药物。
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