Dougan G, Sherratt D J
J Bacteriol. 1977 May;130(2):846-51. doi: 10.1128/jb.130.2.846-851.1977.
Mitomycin C treatment of Escherichia coli K-12 cells containing the nonconjugative plasmid CloDF13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two CloDF13-specified proteins whose molecular weights correspond to cloacin and immunity protein. Five molecules of immunity protein were synthesized for each cloacin DF13 molecule. Mitomycin C-treated cells containing a copy mutant of CloDF13 made three to four times as much of each protein as cells containing wild-type CloDF13. CloDF13 plasmids that contained the transposon Tn1 were isolated. Two did not induce after mitomycin C treatment, failing both to inhibit host cell synthesis and to produce the two new proteins. In minicells, they showed reduced CloDF13-specified protein synthesis and produced three Tn1-specified proteins.
用丝裂霉素C处理含有非接合性质粒CloDF13的大肠杆菌K-12细胞，导致宿主染色体蛋白质合成受到抑制，同时两种由CloDF13编码、分子量分别对应于cloacin和免疫蛋白的蛋白质合成速率很高。每合成一个cloacin DF13分子就会合成五个免疫蛋白分子。用含有CloDF13拷贝突变体的丝裂霉素C处理细胞，每种蛋白质的合成量是含有野生型CloDF13细胞的三到四倍。分离出含有转座子Tn1的CloDF13质粒。其中两个在丝裂霉素C处理后没有诱导反应，既不能抑制宿主细胞合成，也不能产生这两种新蛋白质。在微小细胞中，它们显示出CloDF13编码的蛋白质合成减少，并产生了三种由Tn1编码的蛋白质。
