未染色涂片是评估细胞学样本充分性的有效工具吗？
Are unstained smears, effective tools in the evaluation of the cytological sample adequacy?
作者信息
A L Hemalatha, M K Umarani, U Asha, M N Gayathri
机构信息
Professor and HOD, Department of Pathology, Mysore Medical College & Research Institute , Karnataka, India.
出版信息
J Clin Diagn Res. 2012 Dec;6(10):1672-4. doi: 10.7860/JCDR/2012/4527.2642. Epub 2012 Nov 19.
BACKGROUND
Cytologists are often under time pressure due to a constant need and a demand for quick despatch of reports, which calls for an early assessment of sample adequacy.
OBJECTIVE
To study whether unstained smears are effective in evaluation of sample adequacy of cytology aspirates.
MATERIALS AND METHODS
The study had 3 groups. Assessment of sample adequacy of cytology aspirates was done on unstained smears in Group I (200 cases), Group II (100 cases out of Group I) and Group III ( 80 cases out of Group II ) by a Professor, Assistant Professor and a junior resident respectively. The results were compared with assessment of adequacy on stained smears and statistical analysis was done.
RESULTS
Evaluation of adequacy of cytology aspirates by examination of unstained and stained smears by 3 observers of varied experience revealed no significant difference between the observers and between the stained and unstained smears (p<0.001).
CONCLUSION
Unstained smear study offered advantages over the stained smears in the evaluation of sample adequacy. Hence, it maybe recommended as a routine practice in cytology clinics.
背景
由于持续存在的需求以及对快速发送报告的要求，细胞学家常常面临时间压力，这就需要对样本充足性进行早期评估。
目的
研究未染色涂片在评估细胞抽吸样本充足性方面是否有效。
材料与方法
该研究分为3组。分别由一位教授、一位助理教授和一名住院医师对第一组（200例）、第二组（从第一组中选取100例）和第三组（从第二组中选取80例）的未染色涂片进行细胞抽吸样本充足性评估。将结果与染色涂片的充足性评估进行比较并进行统计分析。
结果
由3位经验不同的观察者对未染色和染色涂片进行细胞抽吸样本充足性评估，结果显示观察者之间以及染色和未染色涂片之间均无显著差异（p<0.001）。
结论
在评估样本充足性方面，未染色涂片研究比染色涂片具有优势。因此，在细胞诊所中，它可被推荐作为常规做法。
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