Shukla Surabhi, Einstein A, Shukla Abhilasha, Mishra Deepika
Department of Oral Pathology and Microbiology, Chandra Dental College, Barabanki, Uttar Pradesh, India.
Department of Oral Pathology and Microbiology, Rishiraj College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India.
J Oral Maxillofac Pathol. 2015 Sep-Dec;19(3):315-8. doi: 10.4103/0973-029X.174611.
Liquid-based cytology (LBC), recommended in the mass screening of potentially malignant cervical and oral lesions, suffers from high cost owing to the use of expensive automated devices and materials. Considering the need for cost-effective LBC techniques, we evaluated the efficacy of an inexpensive manual LBC (MLBC) technique against conventional cytological technique in terms of specimen adequacy and smear quality of oral smears.
Cytological samples were collected from 21 patients using a cytobrush device. After preparation of a conventional smear, the brush containing the remaining sample was immersed in the preservative vial. The preserved material was processed by an MLBC technique and subsequently, direct smears were made from the prepared cell button. Both conventional and MLBC smears were stained by routine Papanicolaou technique and evaluated by an independent observer for the thickness of the smear, cellular distribution, resolution/clarity of cells, cellular staining characteristics and the presence of unsatisfactory background/artifacts. Each parameter was graded as satisfactory; or satisfactory, but limited; or unsatisfactory. Chi-square test was used to compare the values obtained (significance set at P ≤ 0.05).
MLBC technique produced a significant number of satisfactory smears with regard to cell distribution, clarity/resolution, staining characteristics and background/artifacts compared to conventional methods.
MLBC is a cost-effective cytological technique that may produce oral smears with excellent cytomorphology and longer storage life.
液基细胞学检查（LBC）在潜在恶性宫颈和口腔病变的大规模筛查中被推荐使用，但由于使用昂贵的自动化设备和材料，成本较高。考虑到需要具有成本效益的LBC技术，我们就口腔涂片的标本充分性和涂片质量，评估了一种廉价的手动LBC（MLBC）技术相对于传统细胞学技术的效果。
使用细胞刷装置从21名患者收集细胞学样本。制备传统涂片后，将含有剩余样本的刷子浸入保存瓶中。保存的材料采用MLBC技术处理，随后从制备好的细胞团块制作直接涂片。传统涂片和MLBC涂片均采用常规巴氏染色技术染色，并由一名独立观察者评估涂片厚度、细胞分布、细胞分辨率/清晰度、细胞染色特征以及背景/伪像是否令人满意。每个参数分为满意；或满意但有局限；或不满意。采用卡方检验比较所得值（显著性设定为P≤0.05）。
与传统方法相比，MLBC技术在细胞分布、清晰度/分辨率、染色特征和背景/伪像方面产生了大量令人满意的涂片。
MLBC是一种具有成本效益的细胞学技术，可制作出具有优异细胞形态学且保存期限更长的口腔涂片。
