Department of Environmental Sciences, University of Toledo, Toledo, OH, USA.
J Hosp Infect. 2013 Mar;83(3):253-6. doi: 10.1016/j.jhin.2012.12.009. Epub 2013 Jan 30.
The distribution of staphylococcal assemblages on surfaces in hospital rooms was assessed before and after daily disinfection with quaternary ammonia products. DNA was extracted from enrichment cultures of bacteria, which were swabbed from each of nine surface types, and subjected to analysis by staphylococci-specific, denaturing gradient gel electrophoresis. A genetic marker for Staphylococcus epidermidis/kloosii was detected on all surface types before and after cleaning, whereas markers for Staphylococcus aureus and Staphylococcus lugdunensis were detected on five surface types. Overall, genetic makers for several staphylococci known to colonize and infect humans remained ubiquitous in each room following daily disinfection practices.
评估了每日使用季铵盐产品消毒前后，医院病房表面葡萄球菌组合的分布情况。从每个九种表面类型的拭子中提取细菌的富集培养物的 DNA，并通过葡萄球菌特异性变性梯度凝胶电泳进行分析。在清洁前后的所有表面类型上均检测到表皮葡萄球菌/腐生葡萄球菌的遗传标记，而在五种表面类型上检测到金黄色葡萄球菌和路邓葡萄球菌的标记。总体而言，在每日消毒实践后，每个房间中仍然存在着几种已知定植和感染人类的葡萄球菌的遗传标记。
