Drug Metabolism and Toxicology Unit, Department of Biochemistry, Federal University of Technology, Akure 340001, Nigeria.
Asian Pac J Trop Med. 2013 Mar;6(3):200-4. doi: 10.1016/S1995-7645(13)60023-1.
To investigate the restorative effect of Pterocarpus erinaceus (P. erinaceus) and homopterocarpin, an isoflavonoid isolated from it, on indomethacin-induced disruption in gastric homeostasis in rats.
Adult rats were divided into five groups and fasted for 48 h before treatment. Group 1 received olive oil (vehicle), group 2 received 25 mg/kg indomethacin while groups 3-5 received cimetidine (100 mg/kg), homopterocarpin (25 mg/kg) and P. erinaceus ethanolic stem bark extract (100 mg/kg) respectively. After 1 h, all the groups except group 2 were administered 25 mg/kg of indomethacin. One hour later, the rats were sacrificed and the ulcer index and other gastroprotective indices were evaluated.
Indomethacin caused significant injury to the stomach of the rats as reflected in the ulcer indices (9.0±1.4) as compared with that of control (2.0±0.0). Equally, there were significant increases in gastric acid concentration and malondialdehyde level in the stomachs of the ulcerated animals compared with the control. However mucus content, reduced gluthatione level and gastric pH were significantly reduced in the ulcerated animals compared with the control. Pretreatment with either Pterocarpus bark extract or homopterocarpin reversed the effects of indomethacin on the evaluated parameters.
These results indicate that both homopterocarpin and Pterocarpus extract offered gastroprotection against indomethacin-induced ulcer by antioxidative mechanism and the modulation of gastric homeostasis. The results also suggest that homopterocarpin might be responsible for, or contribute to the antiulcerogenic property of P. erinaceus.
研究鸡翅木（Pterocarpus erinaceus）及其从中分离得到的异黄酮化合物 homopterocarpin 对吲哚美辛诱导的大鼠胃稳态破坏的修复作用。
将成年大鼠分为五组，在治疗前禁食 48 小时。第 1 组给予橄榄油（载体），第 2 组给予 25mg/kg 吲哚美辛，第 3-5 组分别给予西咪替丁（100mg/kg）、homopterocarpin（25mg/kg）和鸡翅木乙醇树皮提取物（100mg/kg）。1 小时后，除第 2 组外，所有组均给予 25mg/kg 吲哚美辛。1 小时后，处死大鼠，评估溃疡指数和其他胃保护指数。
与对照组（2.0±0.0）相比，吲哚美辛使大鼠胃明显受损，溃疡指数（9.0±1.4）显著升高。同样，溃疡动物胃中的胃酸浓度和丙二醛水平也显著升高，而胃粘液含量、还原型谷胱甘肽水平和胃 pH 值则显著降低。与对照组相比，鸡翅木树皮提取物或 homopterocarpin 的预处理均逆转了吲哚美辛对评估参数的影响。
这些结果表明，homopterocarpin 和鸡翅木提取物均通过抗氧化机制和调节胃稳态对吲哚美辛诱导的溃疡提供胃保护。结果还表明，homopterocarpin 可能是鸡翅木抗溃疡作用的原因之一，或对其有贡献。
