Department of Periodontics, Government Dental College and Research Institute, Bangalore 560002, Karnataka, India.
Cytokine. 2013 Mar;61(3):772-7. doi: 10.1016/j.cyto.2012.12.022. Epub 2013 Jan 29.
Obesity is increasing in prevalence worldwide and has emerged as a strong risk factor for periodontal disease. Conversely, the remote effects of periodontal disease on various systemic diseases have been proposed. The aim of this study is to determine the presence of MCP-4 and high sensitivity C reactive protein (hsCRP) levels in gingival crevicular fluid (GCF) and serum in obese and non-obese subjects with chronic periodontitis and to find a correlation between MCP-4 and hsCRP in GCF and serum.
Forty subjects (20 males and 20 females) were selected and divided into four groups (10 subjects in each group), based on clinical parameters: group NOH (non-obese healthy), group OH (obese healthy), Group NOCP (non-obese with chronic periodontitis) and group OCP (obese with chronic periodontitis). The levels of serum and GCF MCP-4 were determined by ELISA and hsCRP levels were determined by immunoturbidimetry method.
The mean GCF and serum concentration of MCP-4 was highest for group OCP followed by group NOCP, group OH (in GCF); group OH, group NOCP(in serum) and least in group NOH. The mean hsCRP concentration was highest for group OCP followed by group OH, group NOCP and group NOH. A significant positive correlation was found between serum and GCF MCP-4 and hsCRP levels.
GCF MCP-4 concentrations increased in periodontal disease compared to health and correlated positively with the severity of disease indicating it as a novel marker of periodontal disease. The serum concentration of MCP-4 was found to be more in obese group as compared to nonobese group indicating it as a marker of obesity. Furthermore, based on the positive correlation of MCP-4 and hsCRP found in this study, it can be proposed that MCP-4 and hsCRP may be the markers linking chronic inflammation in obesity and periodontal disease.
肥胖症在全球的患病率不断增加，已成为牙周病的一个重要危险因素。相反，牙周病对各种系统性疾病的远隔效应也已被提出。本研究旨在确定慢性牙周炎肥胖和非肥胖患者的龈沟液（GCF）和血清中 MCP-4 和高敏 C 反应蛋白（hsCRP）水平，并发现 GCF 和血清中 MCP-4 和 hsCRP 之间的相关性。
根据临床参数选择 40 名受试者（20 名男性和 20 名女性），并将其分为四组（每组 10 名）：非肥胖健康组（NOH）、肥胖健康组（OH）、非肥胖慢性牙周炎组（NOCP）和肥胖慢性牙周炎组（OCP）。通过 ELISA 法测定血清和 GCF 中 MCP-4 的水平，免疫比浊法测定 hsCRP 水平。
OCP 组 GCF 和血清中 MCP-4 的平均浓度最高，其次是 NOCP 组、OH 组（GCF）；OH 组、NOCP 组（血清），最低的是 NOH 组。OCP 组的 hsCRP 浓度最高，其次是 OH 组、NOCP 组和 NOH 组。GCF 和血清中 MCP-4 与 hsCRP 水平之间存在显著正相关。
与健康相比，牙周病患者的 GCF MCP-4 浓度升高，并且与疾病的严重程度呈正相关，提示其为牙周病的一种新标志物。与非肥胖组相比，肥胖组血清中 MCP-4 的浓度更高，提示其为肥胖的标志物。此外，根据本研究中发现的 MCP-4 和 hsCRP 之间的正相关关系，可以提出 MCP-4 和 hsCRP 可能是将肥胖症的慢性炎症与牙周病联系起来的标志物。
