El-Demerdash Fatma, Dewer Youssef, ElMazoudy Reda H, Attia Azza A
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Exp Toxicol Pathol. 2013 Sep;65(6):897-901. doi: 10.1016/j.etp.2013.01.002. Epub 2013 Jan 29.
The widespread of pesticide in public health and agriculture has caused severe environmental pollution and health hazards. Methomyl is used worldwide in agriculture and health programs. Besides its advantages in the agriculture, it causes several toxic effects. In this study, we aimed to investigate the effects of methomyl at different time intervals on lipid peroxidation, reduced glutathione (GSH), total sulfhydryl group (T-SH), antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and histopathological changes in mice kidney. Ten CD-1 mice per group were assigned to one of four treatment groups. Group one served as control while groups 2, 3 and 4 were orally treated with 1mgmethomyl/kg BW for 10, 20 and 30 days, respectively. Methomyl significantly increased lipid peroxidation in kidney as compared to control group. Levels of GSH and T-SH and activities of SOD, CAT and GST were found to be decreased. On the other hand, methomyl significantly increased the levels of urea, uric acid and creatinine in serum. The histological examination of kidney revealed damage involving the entire renal nephrons in both 20 and 30 days of methomyl exposure. Severe dilatation of the cortical tissue, congested glomerulus with swelling of the endothelial cells and degeneration of the epithelium cells lining the tubules were observed. In conclusion, the results suggest that methomyl exposure can cause renal damage, oxidative stress, perturbations in antioxidant defense system and histopathologic changes in mice kidney in a time dependent manner.
农药在公共卫生和农业领域的广泛使用已造成严重的环境污染和健康危害。灭多威在全球范围内用于农业和卫生项目。除了在农业方面的优势外，它还会产生多种毒性作用。在本研究中，我们旨在调查不同时间间隔的灭多威对小鼠肾脏脂质过氧化、还原型谷胱甘肽（GSH）、总巯基（T-SH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）等抗氧化酶以及组织病理学变化的影响。每组10只CD-1小鼠被分配到四个治疗组之一。第一组作为对照组，而第2、3和4组分别口服1mg灭多威/千克体重，持续10、20和30天。与对照组相比，灭多威显著增加了肾脏中的脂质过氧化。发现GSH和T-SH水平以及SOD、CAT和GST的活性降低。另一方面，灭多威显著增加了血清中尿素、尿酸和肌酐的水平。肾脏组织学检查显示，在灭多威暴露20天和30天时，整个肾单位均有损伤。观察到皮质组织严重扩张，肾小球充血，内皮细胞肿胀，肾小管内衬上皮细胞变性。总之，结果表明，灭多威暴露可导致小鼠肾脏肾损伤、氧化应激、抗氧化防御系统紊乱和组织病理学变化，且具有时间依赖性。
