Institute of Clinical Microbiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.
Diagn Microbiol Infect Dis. 2013 Apr;75(4):434-6. doi: 10.1016/j.diagmicrobio.2012.12.006. Epub 2013 Jan 30.
This study aims to assess the diversity of campylobacteria (Campylobacter and Arcobacter) in human fecal samples from patients with diarrhea (n = 140) and asymptomatic controls (n = 116) in Chile, using a combination of traditional culture and molecular methods. The culture methods detected campylobacteria in 10.7% of the patients with diarrhea and in 1.7% of the controls. In contrast, the molecular methods detected campylobacteria more often than the traditional culture, with a prevalence of 25.7% and 5.2%, respectively. The traditional methods only recovered the species Campylobacter jejuni, Campylobacter coli, and Arcobacter butzleri, whereas the molecular methods additionally detected the emergent species Campylobacter concisus and Campylobacter ureolyticus.
本研究旨在评估智利腹泻患者（n=140）和无症状对照者（n=116）粪便样本中弯曲杆菌（空肠弯曲菌和弯曲杆菌属）的多样性，采用传统培养和分子方法相结合的方式。培养方法在 10.7%的腹泻患者和 1.7%的对照者中检测到弯曲杆菌。相比之下，分子方法比传统培养方法更常检测到弯曲杆菌，其流行率分别为 25.7%和 5.2%。传统方法仅能恢复空肠弯曲菌、大肠弯曲菌和鲍氏弯曲杆菌，而分子方法还能额外检测到新兴物种短型弯曲杆菌和脲解弯曲杆菌。
