School of Public Health, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
Daicel Arbor Biosciences, Ann Arbor, Michigan 30606, United States.
Environ Sci Technol. 2024 Jul 23;58(29):13065-13075. doi: 10.1021/acs.est.4c01358. Epub 2024 Jul 11.
is an underappreciated emerging global pathogen, despite growing evidence that it is a major contributor of diarrheal illness. Few studies have investigated the occurrence and public health risks that this organism possesses from waterborne exposure routes including through stormwater use. In this study, we assessed the prevalence, virulence potential, and primary sources of stormwater-isolated in fecally contaminated urban stormwater systems. Based on qPCR, was the most common enteric bacterial pathogen [25%] found in stormwater among a panel of pathogens surveyed, including Shiga-toxin producing (STEC) [6%], spp. [4%], and spp. [<1%]. Concentrations of the bacteria, based on qPCR amplification of the single copy gene , were as high as 6.2 log copies/100 mL, suggesting significant loading of this pathogen in some stormwater systems. Importantly, out of 73 unique stormwater culture isolates, 90% were positive for the putative virulence genes , and , while 50-75% of isolates also possessed the virulence genes , , and . Occurrence of . was most often associated with the human fecal pollution marker HF183 in stormwater samples. These results suggest that may be an important bacterial pathogen in stormwater, warranting further study on the risks it represents to public health during stormwater use.
是一种被低估的新兴全球病原体，尽管越来越多的证据表明它是导致腹泻疾病的主要因素之一。很少有研究调查过这种生物体通过包括暴雨水利用在内的水传播途径所具有的发生和公共卫生风险。在这项研究中，我们评估了在受粪便污染的城市暴雨水系统中，从暴雨水分离出的 的流行率、毒力潜力和主要来源。基于 qPCR，在调查的病原体小组中，是在暴雨水样本中发现的最常见的肠内细菌病原体[25%]，包括产志贺毒素的大肠杆菌（STEC）[6%]、 spp. [4%]和 spp. [<1%]。根据单拷贝基因 的 qPCR 扩增，该细菌的浓度高达 6.2 对数拷贝/100 mL，表明在一些暴雨水系统中存在大量这种病原体。重要的是，在 73 个独特的暴雨水培养分离物中，90%的分离物对假定的毒力基因 和 呈阳性，而 50-75%的分离物还具有毒力基因 、 和 。 的出现通常与暴雨水样本中的人类粪便污染标记 HF183 相关。这些结果表明， 可能是暴雨水中的一种重要细菌病原体，需要进一步研究它在暴雨水利用期间对公共健康构成的风险。
