State Key Laboratory of Food Science and Technology, Nanchang University, No. 235, Nanjing East Road, Nanchang, 330047, Jiangxi, P.R. China; Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
J Dairy Sci. 2013 Apr;96(4):2061-2070. doi: 10.3168/jds.2012-6072. Epub 2013 Jan 30.
Liposomes loaded with positively charged lactoferrin (LF) were prepared from milk fat globule membrane-derived phospholipids using a thin-layer dispersion method. The entrapment efficiency of LF in the liposomes and the stability during in vitro gastrointestinal digestion were characterized and examined using dynamic light scattering, transmission electron microscopy, and PAGE. The entrapment efficiency of LF encapsulated in the liposomes was about 46%. The entrapped LF remained unchanged as a function of time and pepsin concentration when the liposome samples were digested in a simulated gastric environment, suggesting that the liposomes prepared from milk fat globule membrane-derived phospholipids were stable and protected the entrapped LF from pepsin hydrolysis. In simulated intestinal fluid, the entrapped LF was more susceptible to hydrolysis by the protease in pancreatin, as shown by changes in the diameter and membrane structure of the liposomes. The release of free fatty acids from the liposomes during digestion in simulated intestinal fluid revealed that the phospholipids in the liposomes were partly hydrolyzed by pancreatic lipase. It was suggested that liposomes may prevent the gastric degradation of LF and reduce the rate of hydrolysis of LF in intestinal conditions.
采用薄层层析分散法，从乳脂肪球膜衍生的磷脂中制备了负载带正电荷乳铁蛋白（LF）的脂质体。通过动态光散射、透射电子显微镜和 PAGE 对 LF 在脂质体中的包封效率和体外胃肠道消化过程中的稳定性进行了表征和检测。LF 的包封效率约为 46%。当脂质体样品在模拟胃环境中消化时，包封的 LF 随时间和胃蛋白酶浓度的变化保持不变，这表明由乳脂肪球膜衍生的磷脂制备的脂质体是稳定的，能够保护包封的 LF 免受胃蛋白酶水解。在模拟肠液中，由于胰蛋白酶中的蛋白酶的作用，包封的 LF 更容易发生水解，这表现为脂质体的直径和膜结构发生了变化。在模拟肠液中消化过程中从脂质体中释放出的游离脂肪酸表明，部分磷脂在胰脂肪酶的作用下水解。这表明脂质体可能防止 LF 在胃中的降解，并降低 LF 在肠道条件下的水解速率。
