Tokaç Mehmet, Taner Gökçe, Aydın Sevtap, Ozkardeş Alper Bilal, Dündar Halit Ziya, Taşlıpınar Mine Yavuz, Arıkök Ata Türker, Kılıç Mehmet, Başaran Arif Ahmet, Basaran Nursen
Department of Surgery, Ministry of Health Atatürk Training and Research Hospital, 06800 Ankara, Turkey.
Food Chem Toxicol. 2013 Nov;61:28-35. doi: 10.1016/j.fct.2013.01.015. Epub 2013 Jan 29.
Curcumin, a most active antioxidant compound, has been suggested to have potential beneficial effects against most metabolic and psychological disorders, including cholestasis. In the present study, the effects of curcumin against oxidative stress and DNA damage induced by bile duct ligation (BDL) in Wistar albino rats for 14 days were investigated. The rats were divided into three following groups: Sham group, the BDL group and the BDL+curcumin group. A daily dose of 50mg/kg curcumin was given to the BDL+curcumin group intragastrically for 14 days. The biomarkers of hepatocellular damage were decreased in the BDL+curcumin group compared to the BDL group, indicating that curcumin recovered the liver functions. DNA damage as assessed by the alkaline comet assay was also found to be low in the BDL+curcumin group. Curcumin significantly reduced malondialdehyde and nitric oxide levels, and enchanced reduced glutathione levels and catalase, superoxide dismutase, and glutathione S-transferase enzymes activities in the livers and kidneys of BDL group. Curcumin treatment in BDL group was found to decrease tumor necrosis factor-alpha levels in the livers of rats. These results suggest that curcumin might have protective effects on the cholestasis-induced injuries in the liver and kidney tissues of rats.
姜黄素是一种活性极高的抗氧化化合物，已被认为对包括胆汁淤积在内的大多数代谢和心理紊乱具有潜在的有益作用。在本研究中，研究了姜黄素对胆管结扎（BDL）诱导的Wistar白化大鼠氧化应激和DNA损伤的影响，为期14天。大鼠被分为以下三组：假手术组、BDL组和BDL+姜黄素组。BDL+姜黄素组每天经胃给予50mg/kg姜黄素，持续14天。与BDL组相比，BDL+姜黄素组肝细胞损伤生物标志物降低，表明姜黄素恢复了肝功能。通过碱性彗星试验评估的DNA损伤在BDL+姜黄素组中也较低。姜黄素显著降低了BDL组肝脏和肾脏中丙二醛和一氧化氮水平，并提高了还原型谷胱甘肽水平以及过氧化氢酶、超氧化物歧化酶和谷胱甘肽S-转移酶的活性。发现BDL组中姜黄素处理可降低大鼠肝脏中肿瘤坏死因子-α水平。这些结果表明，姜黄素可能对大鼠肝脏和肾脏组织中胆汁淤积诱导的损伤具有保护作用。
