Ara Cengiz, Karabulut Aysun Bay, Kirimlioglu Hale, Coban Sacit, Ugras Murat, Kirimliglu Vedat, Yilmaz Sezai
Department of General Surgery, Inonu University School of Medicine, Malatya, Turkey.
Ren Fail. 2005;27(4):435-40.
BACKGROUND/AIMS: This experimental study was designed to evaluate histological changes of the kidney and renal tissue levels of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) and the effect of resveratrol on these metabolites after bile duct ligation in rats.
Secondary biliary cirrhosis was induced by bile duct ligation for 28 days. Swiss albino rats were divided into three groups. Group 1: Sham (n=7), Group 2: Bile duct ligation (n=7), Group 3: Bile duct ligation plus resveratrol (n=7). Bile duct ligation (BDL) plus resveratrol group received 10 mgr/kg dose of resveratrol intraperitoneally daily throughout 28 days. Kidney tissues were harvested to determine the tissue levels of MDA, GSH, and NO activity. Liver and kidney tissues were removed for light microscopic evaluation.
Cholestasis was determined by biochemical and pathologic examination. In the resveratrol-treated rats, levels of MDA were significantly lower than those of the BDL group (p < 0.04). The levels of GSH in the resveratrol-treated rats were significantly higher than those in the BDL group (p < 0.01). The levels of NO in the resveratrol group were significantly lower than those in the BDL group (p < 0.01).
The present study demonstrates that intraperitoneal administration of resveratrol in bile duct ligated rats maintains antioxidant defenses and reduces kidney oxidative damage. This effect of resveratrol may be useful in the preservation of renal oxidative stress in cholestasis.
背景/目的：本实验研究旨在评估大鼠胆管结扎后肾脏的组织学变化、肾组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）和一氧化氮（NO）的水平，以及白藜芦醇对这些代谢产物的影响。
通过胆管结扎28天诱导继发性胆汁性肝硬化。将瑞士白化大鼠分为三组。第1组：假手术组（n = 7），第2组：胆管结扎组（n = 7），第3组：胆管结扎加白藜芦醇组（n = 7）。胆管结扎加白藜芦醇组在整个28天期间每天腹腔注射10毫克/千克剂量的白藜芦醇。采集肾脏组织以测定MDA、GSH的组织水平和NO活性。取出肝脏和肾脏组织进行光学显微镜评估。
通过生化和病理检查确定胆汁淤积。在白藜芦醇治疗的大鼠中，MDA水平显著低于胆管结扎组（p < 0.04）。白藜芦醇治疗的大鼠中GSH水平显著高于胆管结扎组（p < 0.01）。白藜芦醇组的NO水平显著低于胆管结扎组（p < 0.01）。
本研究表明，在胆管结扎的大鼠中腹腔注射白藜芦醇可维持抗氧化防御并减少肾脏氧化损伤。白藜芦醇的这种作用可能有助于在胆汁淤积中保护肾脏氧化应激。
