DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs. Lyngby, Denmark.
Lab Chip. 2013 Apr 21;13(8):1509-14. doi: 10.1039/c2lc41386h.
Reagent pre-storage in a microfluidic chip can enhance operator convenience, simplify the system design, reduce the cost of storage and shipment, and avoid the risk of cross-contamination. Although dry reagents have long been used in lateral flow immunoassays, they have rarely been used for nucleic acid-based point-of-care (POC) assays due to the lack of reliable techniques to dehydrate and store fragile molecules involved in the reaction. In this study, we describe a simple and efficient method for prolonged on-chip storage of PCR reagents. The method is based on gelification of all reagents required for PCR as a ready-to-use product. The approach was successfully implemented in a lab-on-a-foil system, and the gelification process was automated for mass production. Integration of reagents on-chip by gelification greatly facilitated the development of easy-to-use lab-on-a-chip (LOC) devices for fast and cost-effective POC analysis.
试剂在微流控芯片中的预储存可以提高操作人员的便利性，简化系统设计，降低储存和运输成本，并避免交叉污染的风险。尽管干试剂在侧向流动免疫分析中已经使用了很长时间，但由于缺乏可靠的技术来干燥和储存反应中涉及的脆弱分子，它们很少用于基于核酸的即时检测（POC）分析。在本研究中，我们描述了一种简单而有效的方法，可长时间在芯片上储存 PCR 试剂。该方法基于将 PCR 所需的所有试剂胶凝化，制成即用型产品。该方法在箔片上实验室系统中得到了成功实施，并对胶凝化过程进行了自动化处理，以实现大规模生产。通过胶凝化将试剂集成到芯片上，极大地促进了易于使用的芯片实验室（LOC）设备的开发，可用于快速且具有成本效益的即时检测分析。
