黄鼠冬眠期间大脑中（钠+钾）-ATP酶和镁离子-ATP酶活性的变化。

Variations in (Na+ + K+)-ATPase and Mg2+-ATPase activity of the ground squirrel brain during hibernation.

Charnock J S, Simonson L P

Department of Pharmacology, University of Alberta, Edmonton, Canada.

Comp Biochem Physiol B. 1978;59(3):223-9. doi: 10.1016/0305-0491(78)90251-1.

Abstract

The specific activity of brain (Na+ + K+)-ATPase and Mg2+ -ATPase of the ground squirrel (Spermophilus richardsonii) is significantly increased after long-term hibernation. 2. The markedly non-linear thermal dependence of (Na+ + K+)-ATPase is unchanged during hibernation whereas the near linear thermal dependence of Mg2+-ATPase undergoes minor alteration after prolonged hibernation. 3. The sensitivity of (Na+ + K+)-ATPase to inhibition by ouabain is significantly decreased after 100 days of hibernation as is both the rate and amount of [3H]-ouabain binding. 4. These changes may be related to alteration in the phospholipid matrix of the membrane rather than alteration in the protein structure of the enzyme.

摘要

长期冬眠后，理查森黄鼠（Spermophilus richardsonii）大脑中（Na⁺ + K⁺）-ATP酶和Mg²⁺-ATP酶的比活性显著增加。2. （Na⁺ + K⁺）-ATP酶明显的非线性热依赖性在冬眠期间未发生变化，而Mg²⁺-ATP酶近乎线性的热依赖性在长期冬眠后发生了轻微改变。3. 冬眠100天后，（Na⁺ + K⁺）-ATP酶对哇巴因抑制的敏感性显著降低，[³H]-哇巴因结合的速率和量也降低。4. 这些变化可能与膜磷脂基质的改变有关，而不是与酶的蛋白质结构改变有关。