Ashrafi Samad, Mugniéry Didier, van Heese Evelyn Yj, van Aelst Adriaan C, Helder Johannes, Karssen Gerrit
Plant Protection Service, Wageningen Nematode Collection, P.O. Box 9102, 6700 HC Wageningen, The Netherlands ; Ghent University, Department of Biology, Ledeganckstraat 35, 9000 Ghent, Belgium.
Zookeys. 2012(249):1-26. doi: 10.3897/zookeys.249.4138. Epub 2012 Dec 7.
Meloidoderita salinasp. n. is described and illustrated from the halophytic plant Atriplex portulacoides L. (sea purslane) growing in a micro-tidal salt marsh in the Mont-Saint-Michel Bay in France. This new species is the first member of Meloidoderita Poghossian, 1966 collected from a saline environment, and is characterized by the following features: sedentary mature females having a small swollen body with a clear posterior protuberance; slightly dorsally curved stylet, 19.9 µm long, with posteriorly sloping knobs; neck region irregular in shape and twisted; well developed secretory-excretory (S-E) pore, with markedly sclerotized S-E duct running posteriorly; prominent uterus bordered by a thick hyaline wall and filled with eggs. The adult female transforms into a cystoid. Eggs are deposited in both egg-mass and cystoid. Cystoids of Meloidoderita salinasp.n. display a unique sub-cuticular hexagonal beaded pattern. Male without stylet, pharyngeal region degenerated, S-E duct prominent, deirids small, developed testis 97.5 µm long, spicules 18.4 µm long, cloacal opening ventrally protruded, small phasmids posterior to cloaca opening and situated at 5.9 (3.2-7.7) µm from tail end, and conical tail ending in a rounded terminus marked with one (rarely two) ventrally positioned mucro. Additionally, some young malesof the new species were observed enveloped in the last J2 cuticle. Second-stage juvenile body 470 µm long, with a 16.4 µm long stylet, prominent rounded knobs set off from the shaft, hemizonid anterior and adjacent to S-E pore, small deirids located just above S-E pore level, genital primordium located at 68-77% of body length, phasmids small and located at about 19 µm from tail tip, and tail 38.7 µm long, tapering to finely pointed terminus with a finger-like projection. Phylogenetic analyses based on the nearly full length small subunit ribosomal DNA sequences of Meloidoderita salinasp. n. revealed a close relationship of the new species with Sphaeronema alni Turkina & Chizhov, 1986 and placed these two species sister to the rest of Criconematina.
新种盐生拟盘线虫（Meloidoderita salinasp. n.）是从生长在法国圣米歇尔山海湾微潮盐沼中的盐生植物海滨藜（Atriplex portulacoides L.）中描述并图示的。这个新物种是1966年波戈西安（Poghossian）描述的拟盘线虫属（Meloidoderita）中首个从盐环境中采集到的成员，其特征如下：定居的成熟雌虫身体小且肿胀，后端有明显的突出；口针略向背侧弯曲，长19.9 µm，瘤向后倾斜；颈部形状不规则且扭曲；分泌排泄（S-E）孔发达，S-E管向后明显硬化；子宫突出，有厚的透明壁包围，充满卵。成年雌虫转变为囊状。卵产在卵块和囊状结构中。盐生拟盘线虫新种的囊状结构呈现独特的表皮下六边形串珠状图案。雄虫无口针，咽区退化，S-E管突出，侧器小，发育成熟的精巢长97.5 µm，交合刺长18.4 µm，泄殖孔腹侧突出，小尾感器在泄殖孔开口后方，距尾端5.9（3.2 - 7.7）µm，锥形尾末端呈圆形，有一个（很少有两个）腹侧的小刺。此外，观察到一些新种的年轻雄虫被包裹在最后一龄第二阶段幼虫（J2）的角质层中。第二阶段幼虫体长470 µm，口针长16.4 µm，瘤从针干突出呈圆形，半侧器在前方且与S-E孔相邻，小侧器位于S-E孔水平上方，生殖原基位于体长的68 - 77%，尾感器小，位于距尾尖约19 µm处，尾长38.7 µm，逐渐变细至细尖末端，有一个指状突起。基于盐生拟盘线虫新种几乎全长的小亚基核糖体DNA序列的系统发育分析表明，该新物种与1986年图尔基纳（Turkina）和奇若夫（Chizhov）描述的桤木球线虫（Sphaeronema alni）关系密切，并将这两个物种置于环线虫目（Criconematina）其他物种的姐妹位置。
