Vovlas N, Landa B B, Liébanas G, Handoo Z A, Subbotin S A, Castillo P
Istituto per la Protezione delle Piante, Sezione di Bari: Nematologia Agraria, Consiglio Nazionale delle Ricerche (C.N.R.), Via G. Amendola 165/A, 70126 Bari, Italy.
J Nematol. 2006 Sep;38(3):376-82.
A population of the cystoid nematode Meloidoderita kirjanovae was detected parasitizing water mint (Mentha aquatica) in southern Italy. The morphological identification of this species was confirmed by molecular analysis using the internal transcribed spacer 1 (ITS1) and 5.8S gene sequences of nuclear ribosomal DNA (rDNA), which clearly separated it from the closely related species Meloidoderita polygoni. A phylogenetic analysis of M. kirjanovae with species of related genera was conducted using sequences of the D2-D3 expansion segments of the 28S nuclear ribosomal RNA gene. The resulting phylogenetic tree was congruent with trees from an extended dataset for Criconematina and Tylenchida. The basal position of the genus Meloidoderita together with Sphaeronema within the Criconematina clade in this tree may indicate their close relationships. The anatomical changes induced by M. kirjanovae population from Italy in water mint were similar to those reported for a nematode population infecting roots of M. longifolia in Israel. Nematode feeding caused the formation of a stellar syncytium that disorganized the pericycle and vascular root tissues.
在意大利南部，检测到一群囊状线虫Meloidoderita kirjanovae寄生于水生薄荷（Mentha aquatica）。利用核糖体DNA（rDNA）的内部转录间隔区1（ITS1）和5.8S基因序列进行分子分析，证实了该物种的形态学鉴定，该分析明确将其与近缘物种Meloidoderita polygoni区分开来。利用28S核糖体RNA基因的D2-D3扩展片段序列，对kirjanovae与相关属的物种进行了系统发育分析。所得的系统发育树与来自环线虫亚目和垫刃目的扩展数据集的树一致。在这棵树中，Meloidoderita属与Sphaeronema属在环线虫亚目分支中的基部位置可能表明它们关系密切。意大利的kirjanovae种群对水生薄荷造成的解剖学变化与以色列报道的感染长叶薄荷根的线虫种群造成的变化相似。线虫取食导致形成星状合胞体，扰乱了中柱鞘和根维管组织。
