Reed Kelynne E, Richardson John M
Department of Biology, Austin College, Sherman, TX 75090, USA.
Biochem Mol Biol Educ. 2013 Jan-Feb;41(1):34-43. doi: 10.1002/bmb.20663.
We used the Integrated Microbial Genomes Annotation Collaboration Toolkit as a framework to incorporate microbial genomics research into a microbiology and biochemistry course in a way that promoted student learning of bioinformatics and research skills and emphasized teamwork and collaboration as evidenced through multiple assessment mechanisms. Student teams in microbiology used bioinformatics tools to identify and characterize gene products from Mucilaginibacter paludis necessary for the synthesis of specific amino acids and then designed and carried out growth experiments to determine if the organism could indeed synthesize the amino acids. Students in biochemistry worked to characterize one of the amino acid biosynthetic pathways reconstructed by a previous microbiology class through amplification and cloning of the M. paludis genes and complementation analysis of Escherichia coli mutants.
我们使用综合微生物基因组注释协作工具包作为框架，将微生物基因组学研究融入微生物学和生物化学课程中，以促进学生对生物信息学和研究技能的学习，并通过多种评估机制强调团队合作。微生物学课程中的学生团队使用生物信息学工具来鉴定和表征沼泽粘杆菌合成特定氨基酸所需的基因产物，然后设计并进行生长实验，以确定该微生物是否真的能够合成这些氨基酸。生物化学课程中的学生则通过扩增和克隆沼泽粘杆菌基因以及对大肠杆菌突变体进行互补分析，来表征前一个微生物学班级重建的一条氨基酸生物合成途径。
