College of Medicine, Taif University, Al-Taif, Saudi-Arabia.
PLoS One. 2013;8(1):e55500. doi: 10.1371/journal.pone.0055500. Epub 2013 Jan 30.
Human bocavirus (HBoV) was recently discovered in children with respiratory distress and/or diarrhea. To our knowledge, no previous study has reported the existence of bocavirus in Saudi Arabia. Swabs samples from 80 children with respiratory tract infections were examined for the presence of HBoV. Real-time polymerase chain reaction was used as a sensitive method to detect the HBoV. Direct gene sequencing was used to determine the genotype of the detected virus isolates. HBoV was detected in 22.5% of the examined patients. The NP1 partial gene sequence from all patients showed that the circulated strains were related to HBoV-1 genotype. Most of HBoV infected patients showed evidence of mixed coinfection with other viral pathogens. The current study clearly demonstrated that genetically conserved HBoV1 circulates in Saudi Arabia. Interestingly, most of the HBoV1 infected cases were associated with high rates of co-infections with other viruses.
人博卡病毒（HBoV）最近在患有呼吸窘迫和/或腹泻的儿童中被发现。据我们所知，以前没有研究报告过博卡病毒在沙特阿拉伯的存在。对 80 名患有呼吸道感染的儿童的拭子样本进行了检测，以确定是否存在 HBoV。实时聚合酶链反应被用作一种敏感的方法来检测 HBoV。直接基因测序用于确定检测到的病毒分离株的基因型。在检查的患者中，有 22.5%的患者检测到 HBoV。所有患者的 NP1 部分基因序列表明，流行株与 HBoV-1 基因型有关。大多数感染 HBoV 的患者表现出与其他病毒病原体混合感染的证据。本研究清楚地表明，遗传上保守的 HBoV1 在沙特阿拉伯流行。有趣的是，大多数 HBoV1 感染病例与其他病毒的高合并感染率有关。
