Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People's Republic of China.
PLoS One. 2012;7(9):e44876. doi: 10.1371/journal.pone.0044876. Epub 2012 Sep 11.
Human bocavirus (HBoV) is a novel parvovirus associated with respiratory tract diseases and gastrointestinal illness in adult and pediatric patients throughout the world. To investigate the epidemiological and genetic variation of HBoV in Guangzhou, South China, we screened 3460 throat swab samples from 1686 children and 1774 adults with acute respiratory infection symptoms for HBoV between March 2010 and February 2011, and analyzed the complete genome sequence of 2 HBoV strains. Specimens were screened for HBoV by real-time PCR and other 6 common respiratory viruses by RT-PCR or PCR. HBoV was detected in 58 (1.68%) out of 3460 samples, mostly from pediatric patients (52/58) and inpatient children (47/58). Six adult patients were detected as HBoV positive and 5 were emergency cases. Of these HBoV positive cases, 19 (32.76%) had co-pathogens including influenza virus (n = 5), RSV (n = 5), parainfluenza (n = 4), adenovirus (n = 1), coronavirus (n = 7). The complete genome sequences of 2 HBoVs strains (Genbank no. JN794565 and JN794566) were analyzed. Phylogenetic analysis showed that the 2 HBoV strains were HBoV1, and were most genetically close to ST2 (GenBank accession number DQ0000496). Recombination analysis confirmed that HBoV strain GZ9081 was an intra-genotype recombinant strain among HBoV1 variants.
人博卡病毒（HBoV）是一种新型细小病毒，与全球成人和儿科患者的呼吸道疾病和胃肠道疾病有关。为了研究华南广州地区 HBoV 的流行病学和遗传变异，我们于 2010 年 3 月至 2011 年 2 月期间，筛选了 3460 份来自 1686 名儿童和 1774 名急性呼吸道感染症状成人的咽喉拭子样本，以检测 HBoV，并分析了 2 株 HBoV 全基因组序列。通过实时 PCR 检测了标本中的 HBoV，通过 RT-PCR 或 PCR 检测了其他 6 种常见呼吸道病毒。在 3460 个样本中检测到 58 个（1.68%）HBoV，主要来自儿科患者（52/58）和住院儿童（47/58）。在 6 名成年患者中检测到 HBoV 阳性，其中 5 名为急诊患者。在这些 HBoV 阳性病例中，有 19 例（32.76%）存在共病原体，包括流感病毒（n=5）、呼吸道合胞病毒（n=5）、副流感病毒（n=4）、腺病毒（n=1）、冠状病毒（n=7）。分析了 2 株 HBoV （Genbank no. JN794565 和 JN794566）的全基因组序列。系统进化分析显示，这 2 株 HBoV 为 HBoV1，与 ST2（GenBank 登录号 DQ0000496）最为接近。重组分析证实，HBoV 株 GZ9081 是 HBoV1 变异株中的一种内基因型重组株。
