Jungell P
Department of Oral Surgery, University Central Hospital, Helsinki, Finland.
J Cutan Pathol. 1990 Apr;17(2):72-6. doi: 10.1111/j.1600-0560.1990.tb00059.x.
The present study investigated the nature of basement membrane changes in oral lichen planus. For this purpose antibodies to type IV collagen, laminin and fibronectin were applied in avidin-biotin peroxidase complex staining using a pre-embedding immunoelectron microscopy technique. Immunoreactivity to type IV collagen and laminin was restricted to the sub-basal basement membrane and the basement membrane branches extending into the lamina propria. Immunoreactivity to fibronectin was noted also in the connective tissue as a network-like arrangement of thin strands. Although local activated CDB+ cytotoxic lymphocytes and tissue macrophages may be involved in the local pathogenetic process, these findings suggest that the basement membrane alterations are secondary to pathologic changes in the basal cells.
本研究调查了口腔扁平苔藓中基底膜变化的性质。为此，使用包埋前免疫电子显微镜技术，将抗IV型胶原、层粘连蛋白和纤连蛋白的抗体应用于抗生物素蛋白-生物素过氧化物酶复合物染色。对IV型胶原和层粘连蛋白的免疫反应性仅限于基底膜下和延伸至固有层的基底膜分支。在结缔组织中也观察到纤连蛋白的免疫反应性，呈细束状的网络状排列。虽然局部活化的CD8+细胞毒性淋巴细胞和组织巨噬细胞可能参与局部致病过程，但这些发现表明基底膜改变是基底细胞病理变化的继发结果。
