Jungell P, Konttinen Y T, Nortamo P, Malmström M
Department of Oral and Maxillofacial Surgery, Helsinki University, Finland.
Scand J Dent Res. 1989 Aug;97(4):361-7. doi: 10.1111/j.1600-0722.1989.tb01624.x.
Monoclonal anti-CD4, anti-CD8, and anti-CD18 antibodies were applied in avidin-biotin-peroxidase complex staining using a pre-embedding immunoelectron microscopy technique. Although most of the local T cells in situ were of CD4+ subtype, local CD8+ cells generally had a lower nucleus/cytoplasm ratio and contained more cell organelles than CD4+ cells. This suggests a local activation of CD8+ subpopulation, rather than activation of the numerically predominant CD4+ cells. Topographical analysis disclosed that all lymphocytes, regardless of location, were CD18+ and that most of the CD8+ cells were located subbasally and intraepithelially, whereas CD4+ cells often occurred in small clusters deeper down in the subepithelial lymphocyte-rich band. Furthermore, CD8+ cells were often in close contact with macrophages, whereas CD4+ cells were in some instances in direct contact with plasma cells. This indicates that CD4+ cells may be involved in T cell-dependent B cell-mediated immunoglobulin synthesis, whereas CD8+ cytotoxic lymphocytes and tissue macrophages may be involved in the local pathogenetic process leading to basement membrane alterations.
使用包埋前免疫电子显微镜技术，将单克隆抗CD4、抗CD8和抗CD18抗体应用于抗生物素蛋白-生物素-过氧化物酶复合物染色。尽管原位的大多数局部T细胞是CD4+亚型，但局部CD8+细胞的核质比通常低于CD4+细胞，且细胞器比CD4+细胞更多。这表明CD8+亚群被局部激活，而非数量上占优势的CD4+细胞被激活。拓扑分析显示，所有淋巴细胞，无论位置如何，均为CD18+，且大多数CD8+细胞位于基底膜下和上皮内，而CD4+细胞常成小簇状出现在上皮下富含淋巴细胞带的更深处。此外，CD8+细胞常与巨噬细胞密切接触，而CD4+细胞在某些情况下与浆细胞直接接触。这表明CD4+细胞可能参与T细胞依赖性B细胞介导的免疫球蛋白合成，而CD8+细胞毒性淋巴细胞和组织巨噬细胞可能参与导致基底膜改变的局部致病过程。
