East I J, Fitzgerald C J
CSIRO, Division of Tropical Animal Production, Indooroopilly, Queensland, Australia.
J Helminthol. 1990 Mar;64(1):9-14. doi: 10.1017/s0022149x00011810.
Oesophagostomum radiatum developed to fourth stage larvae after 14 days in vitro culture. However, development was totally inhibited if the standard 50% foetal calf serum in the medium was replaced by newborn calf serum. Inhibition did not occur with serum from cattle immune to O. radiatum through natural infection or experimental vaccination irrespective of the titre of specific antibodies to O. radiatum in each serum. The inhibitory action of NCS could be abolished by heat treatment at 56 degrees C for 1 h but not by dialysis or repeated freeze-thawing. The inhibition was not consistent with observed differences in the activity of 19 enzymes in the various sera or the absence of various thiol-containing stimulants of worm development.
辐射食道口线虫在体外培养14天后发育为第四期幼虫。然而，如果培养基中标准的50%胎牛血清被新生牛血清取代，发育则完全受到抑制。无论每种血清中针对辐射食道口线虫的特异性抗体滴度如何，来自通过自然感染或实验性接种而对辐射食道口线虫免疫的牛的血清都不会产生抑制作用。新生牛血清的抑制作用可通过56℃热处理1小时消除，但不能通过透析或反复冻融消除。这种抑制作用与在各种血清中观察到的19种酶活性的差异或缺乏各种含硫醇的蠕虫发育刺激物不一致。
