National University of Colombia, Bogotá 11001, Colombia.
Virol Sin. 2012 Feb;27(1):26-37. doi: 10.1007/s12250-012-3221-5. Epub 2012 Jan 22.
Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.
牛疱疹病毒-1（BoHV-1）分布于世界各地，是牛的主要病原体，是多种临床综合征的致病因子。本研究旨在从 29 只免疫抑制动物中分离和鉴定（分子和生物学鉴定）BoHV-1。共获得 18 个分离株，每个分离株均来自不同的动物，如呼吸道和生殖道。在某些情况下，接种后 12 小时可见细胞病变效应，36-48 小时后变得特征明显。对生物学特性进行了评估，并与爱荷华州和科罗拉多-1 参考株进行了比较，发现噬菌斑大小、用 TCID50 和 PFU/mL 测量的病毒滴度以及一步法病毒曲线存在差异。这些结果表明，与参考株相比，一些分离株在体外具有高度类似强毒的行为，潜伏期更短，病毒更快地释放到上清液中，爆发量和病毒滴度更高。通过单层上的 Western blot 测量，BoHV-1 分离株的糖蛋白表达没有差异。此外，使用限制性内切酶分析，大多数病毒被确认为 BoHV-1.1，只有一种被确认为 BoHV-1.2a 亚型。这些发现表明，一些野生型 BoHV-1 分离株可用作开发新单价疫苗的种子。
