Yoneda Kazunari, Sakuraba Haruhiko, Araki Tomohiro, Shibata Takeshi, Nikki Takahiro, Ohshima Toshihisa
Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):134-6. doi: 10.1107/S1744309112051391. Epub 2013 Jan 31.
An NAD(P)(+)-dependent L-serine 3-dehydrogenase from the hyperthermophilic archaeon Pyrobaculum calidifontis was crystallized using the sitting-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 120.81, b = 57.40, c = 56.37 Å, β = 106.88°. Diffraction data were collected to 1.57 Å resolution on beamline NE3A at the Photon Factory. The overall R(merge) was 4.2% and the data completeness was 90.1%.
利用坐滴气相扩散法，以硫酸铵作为沉淀剂，对嗜热古菌嗜热栖热放线菌（Pyrobaculum calidifontis）中一种依赖NAD(P)(+)的L-丝氨酸3-脱氢酶进行了结晶。晶体属于单斜晶系空间群C2，晶胞参数为a = 120.81，b = 57.40，c = 56.37 Å，β = 106.88°。在光子工厂的NE3A光束线上收集了分辨率为1.57 Å的衍射数据。整体合并R值为4.2%，数据完整性为90.1%。
