甲状腺切除鸡脂肪肝中特异性表达的NAD(P)H依赖性羰基还原酶的表达、纯化、结晶及初步X射线分析。
Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver.
作者信息
Yoneda Kazunari, Fukuda Yudai, Shibata Takeshi, Araki Tomohiro, Nikki Takahiro, Sakuraba Haruhiko, Ohshima Toshihisa
机构信息
Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1568-70. doi: 10.1107/S1744309112046453. Epub 2012 Nov 28.
Abstract
An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27 Å, β=119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.
摘要
一种在甲状腺切除的鸡脂肪肝中特异性表达的NAD(P)H依赖性羰基还原酶，采用坐滴气相扩散法，以聚乙二醇300作为沉淀剂进行结晶。晶体属于单斜晶系空间群C2，晶胞参数为a = 104.26、b = 81.32、c = 77.27 Å，β = 119.43°，在光子工厂的NE3A光束线上衍射至1.86 Å分辨率。整体合并R因子为5.4%，数据完整性为99.4%。
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本文引用的文献
1
iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM.iMOSFLM：一种用于MOSFLM衍射图像处理的新图形界面。
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):271-81. doi: 10.1107/S0907444910048675. Epub 2011 Mar 18.
2
Use of polymerase chain reaction for the rapid construction of synthetic genes.利用聚合酶链反应快速构建合成基因。
Methods Mol Biol. 1993;15:263-8. doi: 10.1385/0-89603-244-2:263.
3
Molecular basis for peroxisomal localization of tetrameric carbonyl reductase.四聚体羰基还原酶过氧化物酶体定位的分子基础。
Structure. 2008 Mar;16(3):388-97. doi: 10.1016/j.str.2007.12.022.
4
An unbiased cell morphology-based screen for new, biologically active small molecules.一种基于细胞形态学的无偏差筛选新型生物活性小分子的方法。
PLoS Biol. 2005 May;3(5):e128. doi: 10.1371/journal.pbio.0030128. Epub 2005 Apr 5.
5
Structural insights into the neuroprotective-acting carbonyl reductase Sniffer of Drosophila melanogaster.果蝇神经保护作用羰基还原酶Sniffer的结构洞察
J Mol Biol. 2004 Oct 1;342(5):1613-24. doi: 10.1016/j.jmb.2004.08.020.
6
Synthesis of betaine-homocysteine S-methyltransferase is continuously enhanced in fatty livers of thyroidectomized chickens.
Poult Sci. 2003 Feb;82(2):207-13. doi: 10.1093/ps/82.2.207.
7
Crystal structure of the ternary complex of mouse lung carbonyl reductase at 1.8 A resolution: the structural origin of coenzyme specificity in the short-chain dehydrogenase/reductase family.小鼠肺羰基还原酶三元复合物的晶体结构，分辨率为1.8埃：短链脱氢酶/还原酶家族中辅酶特异性的结构起源。
Structure. 1996 Jan 15;4(1):33-45. doi: 10.1016/s0969-2126(96)00007-x.
8
9
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
10
Characterization of multiple forms of carbonyl reductase from chicken liver.鸡肝中多种形式羰基还原酶的特性分析。
Enzyme. 1992;46(4-5):221-8. doi: 10.1159/000468791.
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