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甲状腺切除鸡脂肪肝中特异性表达的NAD(P)H依赖性羰基还原酶的表达、纯化、结晶及初步X射线分析。

Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver.

作者信息

Yoneda Kazunari, Fukuda Yudai, Shibata Takeshi, Araki Tomohiro, Nikki Takahiro, Sakuraba Haruhiko, Ohshima Toshihisa

机构信息

Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1568-70. doi: 10.1107/S1744309112046453. Epub 2012 Nov 28.

DOI:10.1107/S1744309112046453
PMID:23192050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3509991/
Abstract

An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27 Å, β=119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.

摘要

一种在甲状腺切除的鸡脂肪肝中特异性表达的NAD(P)H依赖性羰基还原酶，采用坐滴气相扩散法，以聚乙二醇300作为沉淀剂进行结晶。晶体属于单斜晶系空间群C2，晶胞参数为a = 104.26、b = 81.32、c = 77.27 Å，β = 119.43°，在光子工厂的NE3A光束线上衍射至1.86 Å分辨率。整体合并R因子为5.4%，数据完整性为99.4%。

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