Memorial Sloan-Kettering Cancer Center, Research Engineering Laboratory, New York, New York 10065, USA.
J Biomed Opt. 2013 Jun;18(6):61227. doi: 10.1117/1.JBO.18.6.061227.
Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-×-12 mm² of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approach called "strip mosaicing," which was demonstrated on a 10-×-10 mm² of tissue in 3 min. Here we describe further advances in instrumentation, software, and speed. A mechanism was also developed to flatten tissue in order to enable consistent and repeatable acquisition of images over large areas. We demonstrate mosaicing on 10-×-10 mm² of skin tissue with 1-μm lateral resolution in 90 s. A 2.5-×-3.5 cm² piece of breast tissue was scanned with 0.8-μm lateral resolution in 13 min. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Imaging of tumor margins with strip mosaicing confocal microscopy may serve as an adjunct to conventional (frozen or fixed) pathology for guiding surgery.
共聚焦拼接显微镜是一种用于直接在新鲜切除的组织中对肿瘤边缘进行成像的新兴技术平台，无需进行传统病理学所需的处理。此前，已经证明可以在 9 分钟内在 Mohs 手术切除的 12×12mm²皮肤组织上进行拼接，并检测基底细胞癌边缘。去年，我们报告了一种更快的方法“条带拼接”的可行性，该方法在 3 分钟内在 10×10mm²的组织上进行了演示。在这里，我们描述了仪器、软件和速度方面的进一步进展。还开发了一种使组织变平的机制，以便能够在较大区域上一致且可重复地获取图像。我们展示了在 90 秒内在 10×10mm²皮肤组织上以 1μm 横向分辨率进行拼接的结果。在 13 分钟内以 0.8μm 横向分辨率扫描了 2.5×3.5cm²的乳房组织。快速拼接大面积新鲜组织的共聚焦图像可能提供了一种在床边进行病理学检查的方法。使用条带拼接共聚焦显微镜对肿瘤边缘进行成像可能成为指导手术的传统（冷冻或固定）病理学的辅助手段。
