组织相容性抗原激活的细胞毒性T淋巴细胞。I. 体外产生的杀伤细胞绝对频率的估计。
Histocompatibility antigen-activated cytotoxic T lymphocytes. I. Estimates of the absolute frequency of killer cells generated in vitro.
作者信息
Lindahl K F, Wilson D B
机构信息
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19174.
出版信息
J Exp Med. 1977 Mar 1;145(3):500-7. doi: 10.1084/jem.145.3.500.
Abstract
A sensitive procedure employing limiting dilution of activated lymphocytes and 51Cr release from highly labeled target cells was used to derive minimal estimates of the absolute frequency of cytotoxic T lymphocytes (CTL) present in a population of mouse lymphocytes activated to alloantigens of the major histocompatibility complex in bulk mixed lymphocyte cultures. From this figure (0.7-1.2%), the maximal rate of target cell killing could be calculated to be approximately 4 targets/CTL/hour.
摘要
采用对活化淋巴细胞进行有限稀释以及从高度标记的靶细胞中释放⁵¹Cr的敏感程序，来得出对存在于在大量混合淋巴细胞培养物中被主要组织相容性复合体的同种异体抗原激活的小鼠淋巴细胞群体中细胞毒性T淋巴细胞（CTL）绝对频率的最小估计值。根据这个数字（0.7 - 1.2%），可以计算出靶细胞杀伤的最大速率约为每小时4个靶细胞/CTL。
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