Naruse Rika, Suetsugu Mariko, Terasawa Tomoko, Ito Keishi, Hara Kenji, Takebayashi Kohzo, Morita Kimio, Aso Yoshimasa, Inukai Toshihiko
Department of Internal Medicine, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Japan.
Saudi Med J. 2013 Feb;34(2):135-41.
To examine involvement of oxidative stress in the pathogenesis and vascular complications of diabetes.
This cross sectional study was conducted at the Joint Laboratory Office (JLO), Dokkyo Medical University Koshigaya Hospital, Koshigaya, Japan from April 2010 to December 2011. Fasting glucose, glycosylated hemoglobin (HbA1c), serum lipids, urinary albumin excretion (UAE), ankle brachial index and pulse wave velocity were measured in 51 patients with type 2 diabetes and 20 healthy controls. The fundus oculi and Achilles` tendon reflex were also examined in the patients. Oxidative stress was measured by a reactive oxygen metabolites (ROM) test and antioxidant potency was evaluated by a biological antioxidant potential (BAP) test in the Free Radical Analytical System (FRAS)-4. Superoxide dismutase (SOD) activity was assayed using electron spin resonance (ESR).
Diabetic patients tended to have increased ROM compared with healthy subjects, and ROM showed a marked increase with progression of diabetic retinopathy. A significant reduction of BAP was found in patients who were smokers, and BAP was significantly negatively correlated with UAE (p=0.029). Serum SOD activity significantly decreased with progression of diabetic retinopathy (p=0.017).
The FRAS-4 measurements showed that increased oxidative stress and decreased antioxidative potency are linked to deteriorated blood glucose control, heavy smoking, and progression of retinopathy and nephropathy in patients with type 2 diabetes.
探讨氧化应激在糖尿病发病机制及血管并发症中的作用。
本横断面研究于2010年4月至2011年12月在日本越谷市独协医科大学越谷医院联合实验室办公室进行。对51例2型糖尿病患者和20例健康对照者测量空腹血糖、糖化血红蛋白（HbA1c）、血脂、尿白蛋白排泄率（UAE）、踝臂指数和脉搏波速度。还对患者进行了眼底检查和跟腱反射检查。在自由基分析系统（FRAS）-4中，通过活性氧代谢产物（ROM）试验测量氧化应激，并通过生物抗氧化能力（BAP）试验评估抗氧化能力。使用电子自旋共振（ESR）测定超氧化物歧化酶（SOD）活性。
与健康受试者相比，糖尿病患者的ROM往往升高，并且随着糖尿病视网膜病变的进展，ROM显著升高。吸烟者的BAP显著降低，并且BAP与UAE显著负相关（p = 0.029）。随着糖尿病视网膜病变的进展，血清SOD活性显著降低（p = 0.017）。
FRAS-4测量结果表明，氧化应激增加和抗氧化能力降低与2型糖尿病患者血糖控制恶化、大量吸烟以及视网膜病变和肾病的进展有关。
