Buttery J E, Lim H H, de Witt G F
Clin Chim Acta. 1976 Nov 15;73(1):109-15. doi: 10.1016/0009-8981(76)90311-9.
A new and more reliable way of quantitating lactate dehydrogenase activity, using NADH as a standard in a colorimetric assay, is proposed. Adopting the scheme, we obtained a CV of 2.6% and precision (95% limits) of +/-5.4% for enzyme activities ranging from 110 to 600 U/l. Comparative study against the modified kinetic method of Amador et al. showed excellent correlation, r = 0.99, p less than 0.001. A normal range of 75 to 203 U/l was determined for the proposed scheme.
提出了一种新的、更可靠的定量乳酸脱氢酶活性的方法，该方法在比色测定中以NADH作为标准。采用该方案，对于110至600 U/l的酶活性，我们获得了2.6%的变异系数和±5.4%的精密度（95%置信区间）。与Amador等人改进的动力学方法进行的比较研究显示出极好的相关性，r = 0.99，p < 0.001。所提出的方案确定的正常范围为75至203 U/l。
