Liu Ping, Yu Shao-Shuai, He Xin-Rong
Hainan Branch of Pharmacy, PLA General Hospital, Sanya 572013, China.
Zhongguo Zhong Yao Za Zhi. 2012 Nov;37(21):3307-11.
To observe the effect of traditional Chinese medicine storax on the concentration of combined western medicine sulpiride in brain and blood, discuss the effect of storax in inducing resuscitation and increasing the permeability of the gastrointestinal barrier (GB) and the blood-brain-barrier (BBB), and explore the interaction between storax and sulpiride.
Rats were orally administered with the drugs for one week, probes were implanted in their brains and necks by surgery. After balance for 60 min, brain microdialysis and blood microdialysis were adopted for collect dislysates from blood in right atrum and cerebral hippocampus at time periods of 30, 60, 90, 120, 150, 180 min. The concentration of sulpirde in the samples was detected by RP-HPLC. Statistical approaches were adopted to compare the contents of sulpirde in brain and blood of the two groups.
The sulpiride combined with storax group showed a significant higher concentration of sulpiride than the pure sulpiride group. The pure sulpiride group showed a concentration ratio between sulpiride in brain and blood of 1:0.2; while the sulpiride combined with storax group increased the concentration ratio between sulpiride in brain and blood to 1:0.3. Compared with the pure sulpiride group, the sulpiride combined with storax group showed an increase of concentration by 39% in brain and 69% in blood.
Storax can notably increase the concentration of sulpiride in rat brain and blood, indicating that it can increase the permeation of sulpiride through gastrointestinal barrier and BBB. This study reveals the mechanism of storax in inducing resuscitation by promoting the permeation through gastrointestinal barrier and BBB.
观察中药苏合香对复方西药舒必利在脑内及血液中浓度的影响，探讨苏合香开窍及增加胃肠屏障（GB）和血脑屏障（BBB）通透性的作用，以及苏合香与舒必利之间的相互作用。
大鼠连续一周灌胃给药，手术植入脑探针和颈探针。平衡60分钟后，采用脑微透析和血液微透析技术，分别于30、60、90、120、150、180分钟收集右心房血液和脑海马区的透析液。采用反相高效液相色谱法（RP-HPLC）检测样品中舒必利的浓度。采用统计学方法比较两组大鼠脑内和血液中舒必利的含量。
舒必利与苏合香联用组舒必利浓度显著高于单纯舒必利组。单纯舒必利组脑内与血液中舒必利浓度比为1:0.2；舒必利与苏合香联用组脑内与血液中舒必利浓度比提高至1:0.3。与单纯舒必利组相比，舒必利与苏合香联用组脑内浓度升高39%，血液中浓度升高69%。
苏合香可显著提高大鼠脑内和血液中舒必利的浓度，表明其可增加舒必利透过胃肠屏障和血脑屏障的通透性。本研究揭示了苏合香通过促进胃肠屏障和血脑屏障的通透性来开窍的机制。
