Bradamante S, Barchiesi E, Barenghi L, Zoppi F
C.N.R. e Dipartimemto di Chimica Organica e Industriale, Università di Milano, Italy.
Anal Biochem. 1990 Mar;185(2):299-303. doi: 10.1016/0003-2697(90)90297-m.
31P nuclear magnetic resonance spectroscopy has been applied to quantitate phospholipids in human blood plasma and in separate lipoprotein fractions. The addition of suitable detergents to samples produces an excellent chemical shift dispersion and allows the identification and integration of the peaks of the most important phospholipids. Results are in agreement with those obtained with enzymatic colorimetric and TLC methods: our method is characterized by good accuracy and reproducibility.
磷-31核磁共振波谱法已被用于定量测定人血浆及各个脂蛋白组分中的磷脂。向样品中添加合适的去污剂可产生出色的化学位移分散，并能识别和积分最重要磷脂的峰。结果与酶促比色法和薄层色谱法所得结果一致：我们的方法具有良好的准确性和重现性。
