LMU - Ludwig-Maximilians-Universität Munich, Div. Metabolic and Nutritional Medicine, Dr. von Hauner Children's Hospital, University of Munich Medical Center, 80337, Munich, Germany.
Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain.
Lipids Health Dis. 2019 Jan 22;18(1):20. doi: 10.1186/s12944-019-0957-z.
The aim of this study was to analyse the differences in the phospholipid composition of very low density (VLDL), low density (LDL) and high density lipoprotein (HDL) monolayers in pregnant lean and obese women.
LDL, HDL, and VLDL were isolated from plasma samples of 10 lean and 10 obese pregnant women, and their species composition of phosphatidylcholines (PC) and sphingomyelins (SM) was analysed by liquid-chromatography tandem mass-spectrometry. Wilcoxon-Mann-Whitney U test and principal component analysis (PCA) were used to investigate if metabolite profiles differed between the lean/obese group and between lipoprotein species.
No significant differences have been found in the metabolite levels between obese and non-obese pregnant women. The PCA components 1 and 2 separated between LDL, HDL, and VLDL but not between normal weight and obese women. Twelve SM and one PCae were more abundant in LDL than in VLDL. In contrast, four acyl-alkyl-PC and two diacyl-PC were significantly higher in HDL compared to LDL. VLDL and HDL differed in three SM, seven acyl-alkyl-PC and one diacyl-PC (higher values in HDL) and 13 SM (higher in VLDL). We also found associations of some phospholipid species with HDL and LDL cholesterol.
In pregnant women phospholipid composition differs significantly in HDL, LDL and VLDL, similar to previous findings in men and non-pregnant women. Obese and lean pregnant women showed no significant differences in their lipoprotein associated metabolite profile.
本研究旨在分析正常体重和肥胖孕妇的极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）单层中磷脂组成的差异。
从 10 名正常体重和 10 名肥胖孕妇的血浆样本中分离 LDL、HDL 和 VLDL，并通过液相色谱串联质谱法分析其磷脂酰胆碱（PC）和神经鞘磷脂（SM）的种类组成。采用 Wilcoxon-Mann-Whitney U 检验和主成分分析（PCA），考察代谢物谱在瘦/胖组和脂蛋白种类之间是否存在差异。
肥胖和非肥胖孕妇之间的代谢物水平无显著差异。PCA 成分 1 和 2 可区分 LDL、HDL 和 VLDL，但不能区分正常体重和肥胖妇女。12 种 SM 和 1 种 PCae 在 LDL 中的含量高于 VLDL。相反，在 HDL 中，四种酰基-烷基-PC 和两种二酰基-PC 的含量明显高于 LDL。与 LDL 相比，VLDL 和 HDL 在三种 SM、七种酰基-烷基-PC 和一种二酰基-PC（HDL 中含量较高）和 13 种 SM（VLDL 中含量较高）上存在差异。我们还发现一些磷脂种类与 HDL 和 LDL 胆固醇之间存在关联。
与男性和非孕妇中的先前发现相似，在孕妇中，HDL、LDL 和 VLDL 中的磷脂组成存在显著差异。肥胖和正常体重孕妇的脂蛋白相关代谢物谱无显著差异。
