通过高效液相色谱法将具有一至五个甘露糖残基的低聚甘露糖型糖链分离为其吡啶基氨基衍生物。

Separation of oligomannose-type sugar chains having one to five mannose residues by high-performance liquid chromatography as their pyridylamino derivatives.

作者信息

Oku H, Hase S, Ikenaka T

机构信息

Department of Chemistry, Osaka University College of Science, Japan.

出版信息

Anal Biochem. 1990 Mar;185(2):331-4. doi: 10.1016/0003-2697(90)90303-q.

DOI:10.1016/0003-2697(90)90303-q

PMID:2339788

Abstract

Ten oligomannose-type sugar chains (ManGlcNAc2-Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the component PA-sugar chains by reversed-phase HPLC on a Capcell Pak C18 column according to their chemical structures. The method is useful for studying the substrate specificities of alpha-mannosidases with Man5GlcNAc2-PA as a substrate.

摘要

从各种糖蛋白中制备了十条低聚甘露糖型糖链（ManGlcNAc2-Man5GlcNAc2），并用2-氨基吡啶进行荧光标记。首先通过TSK凝胶酰胺-80柱上的高效液相色谱（HPLC）根据其分子大小将荧光吡啶氨基（PA）-糖链分离为五个级分。然后通过Capcell Pak C18柱上的反相高效液相色谱根据其化学结构将每个级分分离为组分PA-糖链。该方法对于以Man5GlcNAc2-PA为底物研究α-甘露糖苷酶的底物特异性很有用。