通过部分乙酰解对寡甘露糖型糖链进行结构分析的荧光方法。

Fluorescence method for the structural analysis of oligomannose-type sugar chains by partial acetolysis.

作者信息

Natsuka S, Hase S, Ikenaka T

机构信息

Department of Chemistry, Osaka University College of Science, Japan.

出版信息

Anal Biochem. 1987 Nov 15;167(1):154-9. doi: 10.1016/0003-2697(87)90146-1.

DOI:10.1016/0003-2697(87)90146-1

PMID:3434793

Abstract

Fluorescence labeling was used in the analysis of partial acetolysis products of oligomannose-type sugar chains with five to nine mannose residues. The principle of the method was the pyridylamination of fragments obtained by the partial acetolysis of pyridylamino sugar chains and the identification of the fragments with an HPLC apparatus equipped with a fluorescence spectrophotometer. The method was tested by analysis of eight oligomannose-type sugar chains with known chemical structures and was found to be effective for analysis of branching structures with samples of 0.5 nmol.

摘要

荧光标记法用于分析含有5至9个甘露糖残基的低聚甘露糖型糖链的部分乙酰解产物。该方法的原理是对吡啶氨基糖链部分乙酰解得到的片段进行吡啶氨基化，并使用配备荧光分光光度计的高效液相色谱仪对片段进行鉴定。通过对8条化学结构已知的低聚甘露糖型糖链进行分析对该方法进行了测试，结果发现该方法对于0.5 nmol样品的分支结构分析有效。