Kalipci E, Ozdemir C, Oztas H
Nevsehir University, Faculty of Engineering & Architecture, Department of Environmental Engineering , Nevsehir, Turkey.
Biotech Histochem. 2013 May;88(3-4):202-7. doi: 10.3109/10520295.2012.758312. Epub 2013 Feb 11.
We studied the eco-toxic and carcinogenic effects of a commonly used 2,4-D acid iso-octylester herbicide on rat liver and pancreas. The rats in Group 1 were fed a standard feed and the rats in Group 2 were fed with standard feed to which was added 200 mg/kg/day 2,4-D acid iso-octylester for 16 weeks. Azaserine, 30 mg/kg/body weight, was injected into rats of Groups 3 and 4 to investigate the effects of 2,4-D acid iso-octylester on the development of neoplasms. After feeding the rats with neoplasms in Group 4 with food including 200 mg/kg/day 2,4-D acid iso-octylester for 16 weeks, an autopsy was carried out on all animals. We found that 2,4-D acid iso-octylester caused the formation of atypical cell foci (ACF) in the pancreata and livers of rats. ACF that were formed experimentally by exposure to azaserine had increased diameter, volume and number of atypical cell foci/mm(2) and mm(3) after exposure to 2,4-D acid iso-octylester. Our observations indicated that this herbicide potentially is a cancer initiator.
我们研究了一种常用的2,4-滴异辛酯除草剂对大鼠肝脏和胰腺的生态毒性和致癌作用。第1组大鼠喂食标准饲料，第2组大鼠喂食添加了200毫克/千克/天2,4-滴异辛酯的标准饲料，持续16周。给第3组和第4组大鼠注射30毫克/千克体重的重氮丝氨酸，以研究2,4-滴异辛酯对肿瘤发生的影响。在给第4组患有肿瘤的大鼠喂食含200毫克/千克/天2,4-滴异辛酯的食物16周后，对所有动物进行解剖。我们发现，2,4-滴异辛酯导致大鼠胰腺和肝脏中形成非典型细胞灶（ACF）。通过暴露于重氮丝氨酸实验性形成的ACF，在暴露于2,4-滴异辛酯后，非典型细胞灶的直径、体积和数量（每平方毫米和每立方毫米）增加。我们的观察表明，这种除草剂可能是一种癌症引发剂。
