Haugland R A, Schlemm D J, Lyons R P, Sferra P R, Chakrabarty A M
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago 60612.
Appl Environ Microbiol. 1990 May;56(5):1357-62. doi: 10.1128/aem.56.5.1357-1362.1990.
Combined cell suspensions of the 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)-metabolizing organism Pseudomonas cepacia AC1100, and the 2,4-dichlorophenoxyacetic acid (2,4-D)-metabolizing organism Alcaligenes eutrophus JMP134 were shown to effectively degrade either of these compounds provided as single substrates. These combined cell suspensions, however, poorly degraded mixtures of the two compounds provided at the same concentrations. Growth and viability studies revealed that such mixtures of 2,4-D and 2,4,5-T were toxic to AC1100 alone and to combinations of AC1100 and JMP134. High-pressure liquid chromatography analyses of culture supernatants of AC1100 incubated with 2,4-D and 2,4,5-T revealed the accumulation of chlorohydroquinone as an apparent dead-end catabolite of 2,4-D and the subsequent accumulation of both 2,4-dichlorophenol and 2,4,5-trichlorophenol. JMP134 cells incubated in the same medium did not catabolize 2,4,5-T and were also inhibited in initiating 2,4-D catabolism. A new derivative of strain AC1100 was constructed by the transfer into this organism of the 2,4-D-degradative plasmid pJP4 from strain JMP134. This new strain, designated RHJ1, was shown to efficiently degrade mixtures of 2,4-D and 2,4,5-T through the simultaneous metabolism of these compounds.
能够代谢2,4,5-三氯苯氧乙酸（2,4,5-T）的洋葱伯克霍尔德菌AC1100与能够代谢2,4-二氯苯氧乙酸（2,4-D）的嗜碱产碱菌JMP134的混合细胞悬液，已证明能有效降解作为单一底物提供的这两种化合物中的任何一种。然而，这些混合细胞悬液对以相同浓度提供的这两种化合物的混合物降解效果不佳。生长和活力研究表明，2,4-D和2,4,5-T的这种混合物单独对AC1100以及AC1100和JMP134的组合都有毒性。对用2,4-D和2,4,5-T培养的AC1100的培养上清液进行高压液相色谱分析，结果显示氯代对苯二酚作为2,4-D明显的终末分解代谢物积累，随后2,4-二氯苯酚和2,4,5-三氯苯酚都积累。在相同培养基中培养的JMP134细胞既不分解代谢2,4,5-T，在启动2,4-D分解代谢时也受到抑制。通过将来自JMP134菌株的2,4-D降解性质粒pJP4转入AC1100菌株，构建了AC1100的一个新衍生物。这个新菌株命名为RHJ1，已证明能通过同时代谢这两种化合物有效降解2,4-D和2,4,5-T的混合物。
