College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710062, PR China.
Carbohydr Polym. 2013 Feb 15;92(2):1197-202. doi: 10.1016/j.carbpol.2012.10.073. Epub 2012 Nov 3.
In this study, response surface methodology was employed to optimize the extraction process of crude polysaccharides from Pomegranate peel with water. Three independent and main variables, including extraction time (h), extraction temperature (°C) and ratio of water to raw material (ml/g), which were of significance for the yields of polysaccharides were studied and the Box-Behnken design was based on the results of a single-factors test. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 1.9h, extraction temperature 98 °C, ratio of water to raw material 37 ml/g. Under the optimization conditions, the experimental yield was 10.358%, which was well matched with the predictive yield of 10.423%.
在这项研究中，采用响应面法对水提法从石榴皮中提取粗多糖的工艺进行了优化。三个独立的主要变量，包括提取时间（h）、提取温度（°C）和水与原料的比例（ml/g），对多糖的得率有重要影响，在此基础上进行了 Box-Behnken 设计，并基于单因素试验的结果。采用多元回归分析对实验数据进行拟合，并使用适当的统计方法进行检验。最佳提取条件为：提取时间 1.9h、提取温度 98°C、水与原料的比例 37ml/g。在优化条件下，实验产率为 10.358%，与预测产率 10.423%吻合较好。
