Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, PR China.
Int J Biol Macromol. 2012 Nov;51(4):523-30. doi: 10.1016/j.ijbiomac.2012.06.006. Epub 2012 Jun 21.
An ultrasonic-assisted procedure for the extraction of polysaccharides from the fruiting body of Hohenbuehelia serotina was investigated using response surface methodology. The effects of four factors on the yield of polysaccharides were studied. The optimized conditions were extraction temperature 94 °C, extraction time 3.0 h, ratio of water to raw material 110:1 and ultrasonic power 480 W. Under these conditions, the experimental yield of polysaccharides was 17.45±0.18%, which was well matched with the predictive yield of 17.54%. The molecular weight of polysaccharide was ranged from 1.19 × 10³ to 1.55 × 10⁴ Da. The polysaccharides were composed of ribose, arabinose, mannose, glucose and galactose in a ratio of 0.65:0.69:9.35:14.24:5.47. Then, the structural features of untreated materials, hot water extraction residue and ultrasonic-assisted extraction residue were compared by SEM. Results indicated that ultrasonic-assisted extraction technology could be an effective and advisable technique.
采用响应面法研究了超声辅助提取 Hohenbuehelia serotina 子实体多糖的方法。研究了四个因素对多糖产率的影响。优化条件为：提取温度 94°C，提取时间 3.0 h，水与原料比 110:1，超声功率 480 W。在此条件下，多糖的实验产率为 17.45±0.18%，与预测产率 17.54%吻合较好。多糖的分子量范围为 1.19×10³~1.55×10⁴ Da。多糖由核糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，比例为 0.65:0.69:9.35:14.24:5.47。然后，通过 SEM 比较了未处理材料、热水提取残渣和超声辅助提取残渣的结构特征。结果表明，超声辅助提取技术是一种有效且可行的技术。
